A simple, sensitive, and precise reversed-phase high performance liquid chromatographic (HPLC) method has been developed, validated and used for simultaneous quantitative determination of cinnamaldehyde and methyl eugenol from the methanolic extract of dried bark powder of Cinnamomum zeylanicum Blume (family Lauraceae). The ultrasonic extraction method was used for the extraction of these compounds. The reversed-phase HPLC analysis was carried out using a Intersil ODS-3V-C(18) (150 mm x 4.6 mm, 5 microm) column and a mobile phase comprising of methanol-acetonitrile-water in the volume ratio of 35:20:45, delivered at a flow rate of 1.0 cm(3)/min. The detection and quantitation of both the compounds was carried out at 221 nm.
A sensitive and accurate high-performance thin-layer chromatographic method has been developed, validated, and used for quantification of dopamine in dried whole-plant powder of Portulaca oleracea Linn. The powder was extracted with 0.1 M HCl and the extract was separated on aluminum HPTLC plates coated with silica gel 60 F 254 , with n-butanol-glacial acetic acid-distilled water 7.0:2.0:1.0 (v/v) as mobile phase. Detection and quantitation were performed by densitometry, with a deuterium lamp, at 280 nm. The response to dopamine reference standard was linear in the concentration range 45.0 to 110.0 μg cm -3 . The validated method was used for quantitative analysis of dopamine in Portulaca oleracea Linn and can be used for routine quality-control analysis of whole-plant powder of Portulaca oleracea Linn.
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