Background: Periodontal disease are common dental and oral health problems in the community. Porphyromonas gingivalis (Pg) is one of the main causes of the periodontal disease. The alveolar bone resorption could be as severity indicator of the disease, so that need a material that can help a process of bone remineralization. Osteoblasts are bone-forming cells that are responsible for the mineralization of the bone matrix. Purple leaf is one of the thirteen commodities developed by DitjenPOM as a superior medicinal plant. Purpose: To determine the effect of 2,5%, 5% and 10% purple leaf extract (EDU) of osteoblasts cells in wistar rats induced by Pg. Method: Thirty male wistar rats divided into 5 groups, namely N (without treatment), K (Pg), P (Pg+EDU 2,5%), P2 (Pg+ EDU 5%), P3 (Pg+EDU 10%). EDU administration once a day for 7 days. The mice were decaputated on the 7 th day and the left mandible was taken to make preparations followed by HE staining, observation and calculation of the number of osteoblast cells in 3 different fields. The results of the observations (data) were added up, averaged and analyzed by One Way ANOVA. Result: The group induced by Pg and EDU had a higher average number of osteoblasts than the K and N group. Conclusion: Purple leaf extract (EDU) can increase osteoblasts cells in the alveolar bone of male wistar rats induced by Pg with the highest number of osteoblasts in the 10% concentration.
Background: The prevalence of periodontal disease ranks second in dental and oral diseases after caries. Periodontitis is inflammation with bacteria infecting the host and involving all parts of the periodontal tissue. If this condition is left untreated, it can lead to fibrosis and irreversible damage. Various types of periodontitis therapy have not been able to provide optimal results in healing periodontitis and that therapy can cause side effects. Because of this background, the researchers wanted to carry out research on alternative treatments for periodontitis with purple leaves as an anti-inflammatory with an indicator of an increase in the number of fibroblast cells in inflamed areas. The use of purple leaves has been used since ancient times for the treatment of wounds and inflammation. Objective: To determine the effect of purple leaf extract (EDU) on increasing the number of fibroblast cells in the gingiva of Wistar rats infected by Porphyromonas gingivalis (Pg). Methods: 30 Wistar rats were divided into 5 groups, namely the normal group (KN), the control group Pg induced (K +) and the treatment group using EDU 2.5% (P1), EDU5% (P2), EDU10% (P3 ). All groups were induced by Pg except KN. EDU administration once a day for 7 days. On the 7th day the rats were decaputed and their gingivae were taken to make preparations and HE staining was carried out. Results readings in 3 different viewpoints were averaged and analyzed by one-way ANOVA. Results: The results showed that the K + group, 2.5% EDU, 5% EDU and 10% EDU groups increased the number of fibroblast significantly when compared to the Pg group (p < 0.05). Conclusion: EDU can increase the number of fibroblasts in Pg-induced mice
Context: Tuberculosis is an infectious disease that highly depends on the immune response. Purple leaves (Graptophyllum pictum (L) Griff) has an immune-modulatory activity. Aims: The purpose of this study was to analyze the effect of purple leaf methanol extract (EMDU) on the expression of IFN-γ in mice lung tissue infected with Mycobacterium tuberculosis. Materials and Methods: M. tuberculosis was infected in mice. The EMDU was given with dose (1.703, 3.406, 6.812) mg kg -1 BW -1 for 14 d after infection. The expression of IFN-γ protein (expression obtained from mice's lung tissue) was examined using immunohistochemical examination using IFN-γ anti monoclonal antibodies. Calculations performed on immunoreactive cells showed positive expression and reddish-brown appearance on the cytoplasm. Calculated as many as ten fields of view using a light microscope at 400 times magnification, then the mean value is taken. The mean value of the number of immunoreactive cells is included as data. Data analysis by one way ANOVA and Duncan test. Results: The treatment group showed that IFN-γ expression in mice tuberculosis was significantly increased (p < 0.05) of EMDU. Conclusions: Administration of EMDU increase of IFN-γ expression in mice tuberculosis.
Background: Tooth extraction is tooth removal procedure from the alveolar socket. Tooth extraction causes wounds in the form of exposed alveolar bone to the oral cavity where the treatment should be observed. Wound healing is begun by an inflammatory phase. The first defense cells that come to the wound tissue are neutrophils. The excessive post-traumatic inflammation can lead neutrophils to be overactivated. This condition may prolong and aggravate the inflammatory response. This study is to determine the effect of cocoa seed extract gel to the number of neutrophil. Method: This research was an experimental research laboratories with the post test control group design. 24 male Wistar rats were used to be the sample. The sample were divided into 2 groups which were control and treatment group. Cocoa seed extract gel was given to the treatment group, whereas the control group didnt given the cocoa seed extract gel. The treatment and control groups were performed decaputation on the 1st, 3rd, and 7th days after tooth extraction. After decaputation was performed, socket tissue was taken to make histological preparation which was stained by Hematoxylin Eosin. Result: The neutrophils average of the treatment group is lower than control group in the 1st, 3rd, and 7th day. Conclusion:The result of the study showed that the 8% cocoa (Theobroma cacao L.) bean gel extract could reduce the neutrophils in rat post-extraction tooth socket.
Periodontitis merupakan penyakit inflamasi pada jaringan periodontal. Porphyromonas gingivalis merupakan salah satu bakteri patogen pemicu periodontitis. Respons awal inflamasi dipicu oleh faktor virulen P.gingivalis antara lain lipopolisakarida (LPS) yang berlanjut pada kerusakan ligamen periodontal sehingga memicu resorpsi tulang alveolar yang diperankan oleh sel osteoklas. Ekstrak daun ungu (Graptophyllum pictum L. Griff) diketahui dapat menghambat P.gingivalis secara in vitro, namun kemampuannya sebagai antiinflamasi dalam menurunkan jumlah sel osteoklas pada kejadian periodontitis belum diketahui. Penelitian ini bertujuan untuk mengevaluasi potensi ekstrak daun ungu (EDU) dalam menurunkan jumlah sel osteoklas pada tikus wistar yang diinfeksi dengan P.gingivalis. Penelitian dilakukan pada tikus wistar yang terdiri atas 6 kelompok. Masing-masing kelompok terdiri dari 5 tikus wistar. Enam kelompok yang terdiri dari kelompok normal (KN): tanpa perlakuan, kelompok kontrol negatif (K-): diinfeksi P.gingivalis (Pg), kelompok kontrol positif (K+): Pg + Tantum Verde, dan 3 kelompok perlakuan yaitu: KP2,5% (Pg + EDU 2,5%), KP5% (Pg + EDU 5%) serta KP10% (Pg + EDU10%). Pemeriksaan histologi dengan pengecatan Haematoxylin-Eosin (HE), jumlah sel osteoklas pada jaringan gingiva, ditentukan dengan menghitung jumlah rata-rata dari 3 lapang pandang tiap potongan jaringan. Analisis data dengan One Way Anova dilanjutkan LSD. Kesimpulan penelitian ini adalah ekstrak daun ungu berpotensi menurunkan jumlah sel osteoklas pada jaringan gingiva tikus wistar (p0,05).
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