The rate of diffusion of chloramphenicol and tetracycline-HCI from white petrolatum wasfoundtoincreaseinthepresenceofeitherwoolwaxesorwool wax derivatives; the effect being more pronounced in conjunction with the latter group. Forbothantibiotics,thereleasingproperties were found tokeep increasing with increasing wax (local Merino) concentration; however, for each antibiotic the increase followsaspecialpattem.Therateof1iberationof the antibiotics could not be correlated to any investigated individual physico-chemical property of the waxes or wax derivatives.
A b s t r a c t -The role displayed by different criteria, to furni.sh the optirrnl experimental conditions, €or controlling the rate of lysis of R1. lysodeikticus substrate suspensions with lysozyme, has been well defined.According to the constructed calibration curve obtained, lysozyrie concentrations, as low as, 0.3 and up to 50 ug/ml could be accurately determined with a high, analytical sensitivity and statistical re1 iability. Statistical analysis of different sets of experiments, carmri ed out at various enzyme concentration levels, in terms of, Lhe relative standard deviation and limit of error at P = 0.99, w e r c found t u bc 2.55 percent and f 0.032, respectively.Evidence on the stability of M. lysodcikticus susp,ensi.ons fur the^-, revealed that, substrate dispersions stored in a refrigerator' show neither any appreciable loss in their, initial absorbance, nor en(. i re original 1yl;ic susceptibility towards lysozpe, within 10 &ys st-orage peri od.
A b s t r a c tAt phys i o Logical hydrogen-i on concen t ra Lion , I)hyctroqhenzon 1, ( 2 esters have been found to exert a synerg.i.stic eyfect: on the lyiic activity of lysozyme; the effect being increased with itncre:ihirig the size of the aliphatic chain. Although the investigated parahens were proved to bind in sipificant quantities with lysozyric., however, such additives fail to mask the formation of s u h s t m t elysozyme complexes. The observation was further extended t LI s u r w organic rnercurials with essentially similar results.At hff'erenl enzyme concentration levels, i,hcnyLrnc>rcuric acetate w a s proved to be the most superior potentidtor, whtreds, methyl paraben &splayed a negligible role.in the light of the findings obtained, the synergistic C f f ' c c l was interpreted on the basis of the preferential orientalion (if the substrate on the enzyme binthng sites. seems to be more accessible in presence of preservdlives. 5uch dn o r i t r i t i i t i t , n Lysozyme is a low molecular weight cationic protein r h c h plays a mjor role in the hygiene of the eye via endowing the Lac1 i n k i l fluids 4 7 3 Copyright 0 1985 by Marcel Dekker, Inc. 0363-9045/85/1 102-047353 S O / O Drug Dev Ind Pharm Downloaded from informahealthcare.com by University of Adelaide on 11/17/14 For personal use only.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.