Insects are commonly utilized in biomedical research and have become increasingly popular in museum collections and as pets. Despite this, objective evaluation of insect euthanasia is scarce. This study investigated the effectiveness of targeted injections of ivermectin or potassium chloride (KCl) for the euthanasia of anesthetized thorny devil stick insects (Eurycantha calcarata). Ten clinically healthy mature insects (six males, four females) were enrolled. Insects were weighed and anesthetized via exposure to a cotton ball soaked with 1.6 mL of liquid isoflurane in a 1 L sealed chamber until loss of righting reflex and response to stimulation (induction). Insects then received one of three treatments: ivermectin 100 mg/kg (n = 4), KCl 200 mEq/kg (n = 4), or 0.9% sodium chloride 100 mL/kg (n = 2) injected along the ventral thoracic midline between the first leg plate and the caudal adjacent plate. Following injection, insects were serially monitored for return of spontaneous movement and righting reflex. Death was defined as the absence of spontaneous movement for 48 h. Median (range) induction time and isoflurane concentration at induction was 36 (22–39) min (n = 9) and 22 (19–22)%, respectively. Euthanasia was successful in 4/4, 3/4, and 0/2 isoflurane-anesthetized insects receiving ivermectin, KCl, or 0.9% sodium chloride, respectively. Recovery was prolonged at 10.5 (sodium chloride female), 11.0 (KCl male), and 18.0 (sodium chloride male) hours. This is the first prospective investigation of euthanasia in adult E. calcarata. In this preliminary study, ivermectin 100 mg/kg via ventral midline injection was effective for euthanasia of thorny devil stick insects.
A wild caught white catfish (Ameiurus catus Linnaeus) developed multiple cutaneous masses. Cytology revealed neoplastic lymphocytes and microscopy confirmed dermal infiltration with epitheliotropism in the epidermis, oral mucosa, and cornea, without internal organ involvement. Transmission electron microscopy did not identify viral particles. Histopathology supported cutaneous epitheliotropic lymphosarcoma, a condition most commonly reported in mammals. This is the first reported case of cutaneous epitheliotropic lymphosarcoma in an ictalurid and one of the few published cases of this condition in any fish species.
When subjected to cold environmental temperatures, cheloniid sea turtles can experience debilitating lethargy, anorexia, and potential mortality in a phenomenon known as cold-stunning. Every year, hundreds to thousands of cold-stunned sea turtles are transported to rehabilitation centers for medical and nutritional care. The objective of this study was to investigate one aspect of nutritional status in cold-stunned sea turtles: fatty acid profiles. Blood was collected from eleven green (Chelonia mydas), twelve Kemp’s ridley (Lepidochelys kempii), and three loggerhead (Caretta caretta) juvenile sea turtles found cold-stunned along the coast of North Carolina, USA. Whole blood (~160 µL) was dried onto specialized paper spot cards, frozen, and subsequently analyzed via gas chromatography to quantify fatty acid percentages. Significant differences among species were identified for 19 out of 36 individual fatty acids analyzed and six out of seven fatty acid groups evaluated (P < 0.5). The whole blood fatty acid profiles of cold-stunned green and Kemp’s ridley sea turtles were similar to prior published profiles of healthy conspecifics. Marginal numerical differences noted upon visual comparison included that cold-stunned sea turtles had lower proportions of total polyunsaturated fatty acids (PUFA) and monoenes and higher proportions of total saturated fatty acids relative to healthy conspecifics. These differences may reflect acute impacts of cold-stunning on circulating plasma fatty acids or may be the result of natural seasonal variations. These data provide practical information to aid in the diet design of sea turtles in rehabilitation settings.
A mature male bowfin fish (Amia calva) presented with a left-sided lateral caudoventral coelomic swelling. Diagnostic imaging (computed tomography and ultrasound) revealed a focal, expansile muscular mass measuring 6.4 × 3.8 cm in diameter and contiguous with the musculature of the left body wall at the level of the pelvic fin. The mass was surgically excised. Histopathology categorized the mass as a malignant melanophoroma. The patient made a full recovery and lived 4 additional years. The patient died from unrelated causes, and necropsy revealed no evidence of recurrence of neoplasia. This is the first documentation of surgical treatment of a melanophoroma in A calva.
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