SummaryThe solvents acetonitrile, methanol, N,N-dimethylformamide, dimethyl sulfoxide, formamide, and deionized water were investigated for their ability to support current flow without added electrolyte. Using open tubular capillary electro horesis, currents were measured to be in the nanoampere (10 A) range for all solvents but formamide (lo4 A). Comparisons with flow data showed no clear relationship between current and electroosmotic flow. Packed capillary columns (3-pm ODs) were used for separations using both pure solvent and hydroorganic mixtures as mobile phases. Separation of four hydrophobic dyes showed mild retention in pure ACN. A 16 polynuclear aromatic hydrocarbon (PAH) standard solution was separated in 80/20 ACNlHzO with reduced plate heights (h) between 2.8 and 3.1 for retained species. A separation of nine androstenediones was achieved using a 70130 MeOWHzO mobile phase.
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