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Linkage of 23 Microsatellite Marker on Chromosome 6 and 7 to Downy Mildew Resistance on Maize. Roberdi, Hajrial Aswidinnoor, Asep Setiawan, Sutrisno, Marcia B. Pabendon, and M. Azrai. Downy mildew caused by Peronosclerospora is one of most important maize disease in several countries, including Indonesia. Parental and progenies selection based on conventional breeding is time consuming and laborious. Development of molecular biology produces many DNA markers used for selection, one of them is microsatellite. The aim of this research to identify microsatellite markers associated with downy mildew resistance on maize progeny MR-4 X AMATLCOHS-9-1-1-1-1-1-2-B, on chromosome 6 and 7. This research was consisted of two activities, phenotypic and genotypic analysis. Phenotypic analysis used 175 progenies BC 1 F 2 and both of parents. This analysis included planting of spreading row, inoculums preparation, inoculation of spreader rows, test material planting, inoculation of test material and observation. Genotypic analysis used 175 progenies BC 1 F 1 and both of parents. This analysis included DNA genome isolation, PCR analysis, electrophoresis, gel staining and data scoring. Percentage of downy mildew infections on MR-4 was 76%, while these on AMATLCOHS-9-1-1-1-1-1-2-B was 16%, and on 175 progenies had range from 10.1-100%. Out of 23 SSR, 12 markers could be mapped in chromosome 6 and 11 markers in chromosome 7. QTL analyses showed that chromosome 7 contain one QTL in position between phi082 and phi116I marker as far as 18.6 cM with 2.6 LOD value.

To select a rehabilitation area of coral reefs in Pahat Island within Anambas Islands, this study was conducted to asses coral reefs condition using diver-towed or manta tow surveys and underwater photo transects (UPT). The results of the manta tow surveys showed coral cover dominated by hard corals percentage ranged between 11% and 50% (categories 2 and 3) in the western, northern and southern parts of Pahat Island. Distribution of hard coral percentage with more than 50% (Category 4: 51% - 75% and 5: 76% -100%) was observed in the eastern part of Pahat Island. While results from UPT showed the hard coral percentage of 62.5%, 33.67% and 17% in the eastern, western and northern of Pahat Island, respectively. There are similarities in the results of coral reef cover Obtained by the manta tow and UPT methods. Conclusions, this study recommends western Pahat Island as a good alternative for a rehabilitation site as the coral coverage was in poor condition.

One of the emerging topics in agriculture is application of biophysical method such as the use of magnetic field (MF) to treat seeds for improved plants production. An experiment has been carried out to determine the effect of MF exposure on sorghum seeds and its subsequent growth in soil. The treatments were different strength of MF (control, 1 mT, 6 mT) and exposure duration (5, 10, 15 min). The magnetically treated seeds showed higher seed germination percentage, vigour index, seed germination index, seedling growth, plant growth and chlorophyll content compared to non-magnetically treated seeds. The best duration of MF exposure was 15 minutes, either at MF strength 1 mT or 6 mT. It is suggested that pre-sowing MF treatment has the potential to improve crop productivity through the enhancement of germination, seedling emergence, and growth of plants.

No abstract

Various reports of molecular genetic diversity evaluation of physic nut (J. curcas) have given inconsistent results. Part of the reasons were because of the used of unrealiable markers. This study was conducted to evaluate genetic diversity of Indonesian physic nut germplasm using four types of molecular markers (RAPD, ISSR, SSR and SCAR markers). Twenty four J. curcas accessions planted in Pakuwon, Sukabumi, with various phenotypes were evaluated. Twenty eight SSR marker loci yielded monomorphic allele pattern and indicated that the evaluated accessions probably were all genetically homogeneous for the respective loci. Eight RAPD and 4 ISSR primers out of the total 31 tested primers produced scoreable markers and some (i.e. UBC 873, OPG 17, OPP 03 and OPQ 11 primers) generated polymorphics markers. Genetic similarity coefficiens among evaluated accessions ranged from 0.6 to 1.0 with a population mean of 0.9 indicating low diversity and narrow genetic background among accessions in all populations. Therefore, breeding program utilizing such population would only result in low genetic gain. Based on the evaluated SCAR markers, all accessions belonged to the non-toxic Mexican type of physic nut. This information is important inputs for designing future physic nut breeding strategies in Indonesia.

Pelaksanaan jenjang karir perawat dimulai sejak adanya regulasi yang mengatur sistem jenjang karir perawat di Indonesia. Penelitian ini bertujuan untuk mengetahui pengaruh faktor individu pasien dan implementasi jenjang karir perawat klinik terhadap kepuasan di Rumah Sakit Pertamina Cirebon. Desain penelitian cross-sectional dengan jenis korelasional. Jumlah sampel sebanyak 96 responden sebagai pasien rawat inap. Pengumpulan data penelitian menggunakan intrumen kuesioner dan wawancara. Analisis data menggunakan uji Spearman’s dan   t-Independent. Hasil penelitian membuktikan bahwa implementasi jenjang karir perawat klinik memberikan pengaruh terhadap dimensi kepuasan pasien dengan p value 0.000. Oleh sebab itu direkomendasikan kepada manajemen Rumah Sakit dalam menerapkan jenjang karir perawat klinik perlu adanya komitmen pimpinan, alokasi biaya, sumber daya perawat yang memadai, peningkatan kesejahteraan perawat dan melengkapi sarana serta pra sarana. INFORMASI TAMBAHAN Catatan PenerbitPolekkes Kemenkes Kendari menyatakan tetap netral sehubungan dengan klaim dari perspektif atau buah pikiran yang diterbitkan dan dari afiliasi institusional manapun. PendanaanPenulis tidak menerima pendanaan yang sifatnya spesifik untuk kajian ini. Konflik KepentinganPenulis tidak memiliki konflik kepentingan. Berbagi DataKuesoioner penelitian dan hasil  uji validitas-reliabilitasnya tersedia secara umum di https://doi.org/10.6084/m9.figshare.12442910

Abstract. Alhamdi MFF, Setiawan A, Ilyas S, Ho WK. 2020. Genetic variability of Indonesian landraces of Vigna subterranea: The morphological characteristics and molecular analysis using SSR markers. Biodiversitas 21: 3929-3937. Bambara groundnut (Vigna subterranea (L.) Verdc.) is a potential grain, which can be considered as an alternative source of protein and carbohydrate. Due to unavailability of commercial bambara groundut cultivar in Indonesia, the characterization of bambara groundnut landraces is an important step before developing cultivar with traits of interest. The objective of the research was to access genetic variability of Indonesian landraces of bambara groundnut with different seed coat colors based on morphological and molecular markers. The experiment was arranged as split-plot in a complete randomized block design with the main plot was cultivation methods and the sub plot was landraces. There were differences in leaf shape and pod shape among the landraces. There were two main clusters of Indonesian landraces of bambara groundnut with 88.28% similarity. The first cluster was Cream, Brown Sumedang, Black Sumedang and Black Tasikmalaya, and the second cluster was Black Sukabumi, Brown Gresik, Black Madura, and Black Gresik. The result based on SSR marker with capillary electrophoresis indicated Black Gresik and Black Madura landraces were different from other Indonesian landraces.  Cream Sumedang or Brown Sumedang from the first cluster and Black Gresik or Brown Gresik from the second cluster have the farthest distances for developing improved variety of bambara groundnut.