It has been confirmed that copper particles can be implanted into a poly(oxymethylene) thermoplastic resin substrate by using a laser-assisted powder jet and copper wiring can be formed. Irradiating the laser on the substrate, copper powder with a particle size of 1 or 1.5 µm was jetted using Ar gas, and was implanted into the substrate. A green laser of CW type with a power of 200 mW and a spot radius of 2.5 mm was condensed at the same spot as the powder jetted on the substrate using a focal length of 50 mm. The smallest wiring width of about 90 µm was formed by the laser-assisted powder jet of 1 µm particles at 0.3 MPa. The depth of the formed wiring increased, and its width and the resistivity decreased, as the number of repetitions of the wiring process increased under the conditions of a powder jet of 0.6 MPa formed using 1.5 µm particles. The smallest resistivity and the largest implantation depth were 0.31 µΩ·m and 75 µm for 5 repetitions, respectively.
Japan General IncorporatedAssociation cettoida] probe atomic force microscopy (AFM). KinA and SpoOF were immobitized on a glass substrate and a glass sphere using Langmuir B]odget films of a chelate amphiphile. The conditions of adsorbing proteins were determined using quartz crystal microbalance and AFM. Surface fbrce protltes for KinA and SpoOF showed only repulsive force in the buffer sotution with/without ATP, Adding cefactor Mg2+ and Ca2+ to buffer so]ution with ATP, attractjve forces and adhesive forces were observed on approach and in retTaction pTocess, These forees were not observed without ATP. These results demonstrated the specific interaction between KinA and SpoOF only in the presence of a substrate ATP and cofactor divaient ions. tPT164 GroELMMsug#asCP86opXFyVF7M-rereasor Functional and Stopped-fiow analysis of circularly permutated GroEL mutant CP86
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