The genetic improvement of Lactococcus lactis is a matter of biotechnological interest in the food industry and in the pharmaceutical and medical fields. However, to construct a food-grade delivery system, both the presence of antibiotic markers or plasmid sequences should be avoided and the maintenance and expression of the cloned gene should be guaranteed. The objective of this work was to produce crossover mutants of L. lactis with a reporter gene under the control of an inducible promoter in order to evaluate the level of gene expression. We utilized a nuclease gene of Staphylococcus aureus as a reporter gene, P(nisA) as the nisin-inducible promoter, a non-essential gene involved in histidine biosynthesis of L. lactis as the site for homologous recombination, and pRV300 as a suicide vector for the genomic integration in L. lactis NZ9000. Single- and double-crossover mutants were identified by genotype and phenotype. Relative to episomal transformants of L. lactis, the level of expression of the heterologous protein after nisin induction was similar in the crossover mutants, suggesting that a single copy of the heterologous gene can be used to produce the protein of interest.
Abstracts(sinuforte) and sinupred were effective mono-therapeutic remedies. In case of complications mucoregulatory preparations are quite effective. Conclusion Thus, effectiveness of monotherapy with the mucoregulatory preparations in case of acute middle otitis is similar to standard treatment and could be regarded as alternative remedy p= 0.01. As for treatment of complicated disease combined treatment significantly reduces treatment duration p<0.001.
OCCULT LUMBAR DERMOID CYST REVEALED BY RECURRENT KLEBSIELLA MENINGITIS
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