No abstract

Introduction: Periodontal ligament plays an essential role in preventing relapse after orthodontic treatment. Hyperbaric Oxygen Therapy (HBOT) and propolis gel can increase the amount of fibroblast in the tension area during orthodontic treatment, thus affecting the periodontal ligament. This research was aimed to analyse the difference of the width of the periodontal ligament and amount of fibroblast in the tension area with the administration of propolis gel and HBOT in an attempt to prevent orthodontic relapse. Methods: Forty-two male guinea pigs were randomly divided into 7 groups of treatments― the untreated group (I – negative group), group with rubber separator (II – positive group), 3% propolis gel treatment group (III), 5% propolis gel treatment group (IV), the HBOT treatment group (V), combination of 3% propolis gel and HBOT treatment group, and combination of 5% propolis gel and HBOT treatment group. The upper left central incisor was extracted distally using a 14-days separator rubber in the positive group and the treatment group; then the separator rubber was removed for 2 days to conduct the relapse process. The data were analysed by LSD statistical test. Results: The result of the combination of HBOT and propolis gel treatment showed significant differences among all groups (p<0.05) in the width of the periodontal ligament (1.03), and the number of fibroblasts was 95.67 in the tension site. Conclusions: The combination of HBOT and propolis gel affect the width of periodontal ligament and the number of fibroblasts in the area of the orthodontic relapse.

Background: Orthodontic tooth movement is a continual and balanced process between bone deposition and bone resorption in pressure and tension sites. Stichopus hermanii is one of the best fishery commodities in

Context: Orthodontic force causes the compression of the periodontal ligament in inhibiting vascularization on the pressure side of orthodontic tooth movement (OTM), while on the opposite side, the periodontal ligament is stretched. Hyperbaric oxygen therapy (HBOT) can increase the dissolved oxygen in the blood, which can affect the tissue regeneration of the periodontal ligament. Aims: To evaluate the effect of HBO 2.4 absolute atmospheres (ATA) for 3 × 30 minutes with 5 minutes interval for 7 days as an adjuvant therapy to accelerate periodontal ligament remodeling during OTM in Cavia cobaya. Methods: The study was an experimental laboratory research with a completely randomized design. Twenty-four males C. cobaya were divided into three groups, (X1) as the control group, (X2) as the group with orthodontic force, and (X3) as the group with orthodontic force followed by HBO 2.4 ATA. Matrix Metalloproteinase (MMP)-8 and Transforming Growth Factor (TGF)-β expressions were measured by using immunohistochemistry. OTM was then examined by applying linear distance and analyzed by employing ratio test (p<0.05). Results: Ratio analysis shows HBO 2.4 ATA for 3 × 30 minutes once a day with 5 minutes intervals for 7 days has increased MMP-8 expressions in the pressure area and TGF-β in the tension area (p<0.05). Conclusions: HBO 2.4 ATA for 3 × 30 minutes once a day for 7 with 5 minutes intervals is able to effectively accelerate the periodontal ligament remodeling process expressed by the increased expression MMP-8, especially within the pressure area and TGF-β in the tension area.

No abstract