a b s t r a c t Jatropha curcas l. (euphorbiaceae) is an important plant. it has been used as pesticide, and herbal medicine. the oil from J. curcas can serve as biofuel that have high economically value. this plant contain toxic compound such as phorbol ester. reducing of toxic compound from the plant would be useful to give added values of this plant. callus cultures were initiated from J. curcas l. seed using Ms medium, and treated by manganese chloride and n-ethylmaleimid. Phorbol ester content from seeds, callus and treated callus was measured by high performance liquid chromatography (HPlc). content of phorbol ester in seed and callus culture were 7,41 mg/g DW and 2,44 mg/g DW -5,27 mg/g DW, respectively. treatment using manganese chloride (Mncl 2 ) 2 mM and 3 mM showed that the content of phorbol ester in callus culture decreased up to 30,5% and 30,6% respectively, at day 7. While, treatment using n-ethylmaleimid (neM) 0,6; 0,9; and 1,2 mM showed that the content of phorbol ester in callus culture decrease up to 26,6%, 6,25%, and 32,2% respectively, at day 21. Phorbol ester content in callus culture of J. curcas was lower than its original seeds then it can be more reduced using manganese chloride and n-ethylmaleimid. This finding showed that cell cultures technique can be used to reduce the production of toxic compounds in J. curcas
ELFAHMI , ARTRI , KOMAR RUSLAN belongs to the Euphorbiaceae family which has a high economic value as a source of biofuel.. This plant has been reported containing toxic compounds such as curcin and phorbol ester and its derivatives which may become a problem if is processed to biofuel. In order to investigate the chemical constituents of this plant, a research on phytochemical and initiation of cell and organ culture has been carried out. collected from different regions in Indonesia showed containing relatively the same profile of chemical contents. Dominant compounds that were detected by GCMS are hydrocarbon such as 2-heptenal, decadienal, hexsadecane, pentadecane, cyclooctane etc, fatty acid such as octadecanoate acid, ethyl linoleate, ethyl stearate, hexadecanoate acid and steroid such as stigmasterol, fucosterol, sitosterol. No phorbol ester and its derivatives have been detected yet by the GCMS method. Callus and suspension cultures of have been established using Murashige and Skoog medium supplemented with 4 % (w/w) sucrose, solidified with 0.9% agar and growth hormon NAA 2 mg/L : BAP 0,5 mg/L.
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