Human alpha Interferons (hIFNα) have been shown to have antiviral, antiproliferative and immunomodulatory activities. The human interferon alpha2b (hIFNα2b), is one of the human interferon alpha2 sub variants, naturally synthesized as a polypeptide of 188 amino acid residues, the first 23 residues of which represents a signal peptide. In the present study, the hIFNα2b gene was expressed after being fused with Glutathione S-Transferase (GST) gene. The hIFNα2b gene was amplified from human genomic DNA by using a pair of specific primers, cloned into an Escherichia coli expression vector and expressed in E. coli cells under the direction of the tac promoter. The expressed protein was purified using a one-step affinity chromatography column containing immobilized gluthatione-bound resin. The purified protein was shown to react specifically with anti-human-interferon-alpha antibody, confirming that the protein was the human interferon alpha molecule. This strategy has the potential to be used as an alternative mean for production of pure human interferon α proteins for therapeutic purposes and for further studies on their molecular characterization and mechanism of action.
Net primary production (NPP) is one of the approaches used to estimate the amount of carbon sequestration by plants. This research aims to estimate the total carbon flux exchanged from different ages of oil palm using remote sensing. The study site was at the PTPN VI Batang Hari, Jambi, Sumatra, Indonesia. The amount of carbon sequestration by oil palm plantations at PTPN VI Batang Hari, Jambi can be estimated using remote sensing based on the light use efficiency (LUE) model. The results showed that the oil palm age affects the amount of carbon sequestrated. The lowest Net primary production value was found at one year of planting 4.28 gCm-2day-1, and the highest was 9.38 gCm-2day-1 at 20 years of planting. The model LUE output was validated using Eddy covariance data and the results showed a low error and a high accuracy rate with RMSE = 0.05 gCMJ-1, R2 = 92%, and p-value = 0.04. We concluded that the LUE model can be used with high accuracy to estimate the amount of carbon absorption of oil palm when direct measurement is unavailable.
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