An elution electrophoresis system in which a porous packed bed is used for separation and a flow photometer or colorimeter for continuous monitoring of the eluate may be capable of rapid, high-resolution analysis of serum proteins and other protein mixtures with very little manual labor. In a prototype of such a system we used a cooled separations column, 3 mm in diameter and 40 cm long, containing polyacrylamide beads. Samples are introduced, via a microsyringe, through a septum at the column midpoint. Typical analyses for serum proteins or serum isoenzymes require an electrophoresis time of about 30 min at 1200 V.
Bei der Behandlung mit wäßriger Essigsäure erhält man aus der 5‐S‐Acetyl‐ 3,6‐di‐O‐benzyl‐1,2‐O‐isopropyliden‐5‐thio‐a‐D‐glucofuranose (I), die in bekannter Weise hergestellt wurde, 78% der Glucofuranose (II) und 16% der Thioglucopyranose (III).
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