Natural saltlicks are used by mammal species mainly to supplement mineral deficiency playing critical role in animal ecology. There is information gap on the use of natural saltlicks by mammals in Bhutan. Nine natural saltlicks from Jomotsangkha Wildlife Sanctuary were purposively selected to fill this gap of information. The study aimed to assess mineral composition and ecological behaviour of mammals at natural saltlicks. Nine composite soil samples were randomly collected and nine camera traps were set up at nine saltlicks for a duration of 56 days from 2 January to 28 February, 2019. Data management and analysis were carried out using camerabase and R software. Potassium, phosphorus and sodium elements were found in the saltlicks. Camera traps yielded 419 independent events of 12 species under 10 families. Herbivores were most common (n = 390) and non-herbivores the least (n = 12). Wild dog was also captured licking salts (n = 1) which is least reported across the world. Mineral composition (r = 0.70, p < .05) and anthropogenic activities (r = 0.60, p < .05) were key factors affecting the visitation rate and ecological behaviour of mammal species. Disturbed saltlicks from Samdrupcholing Range revealed fewer individuals of mammals (n = 71) with disturbed ecological behaviour while undisturbed saltlicks from Jomotsangkha Range revealed higher individuals of mammals (n = 340) with undisturbed ecological behaviour. Therefore, anthropogenic activities at disturbed saltlicks call for planned monitoring.

Orchids are the largest and diverse families of flowering plants. Orchids are found growing mostly in tropical climates affecting various communities socio-economically. Among many protected areas in Bhutan, Jomotshangkha Wildlife Sanctuary (JWS) is the least explored in terms of flora and fauna. The study was carried out to assess diversity, host tree preferences and to determine the growth zone of epiphytic orchids along the altitudinal gradient in Langchenphu gewog in JWS. Transect technique was used in which three trails transect were laid out with 10 sampling plots in each transect. Plots size of 20 m × 20 m along the altitudinal gradients were established maintaining an altitudinal interval of 100 meters between each sample plot. A total of 42 species of epiphytic orchids from 20 genera was recorded of23 host tree species under 15 families were recorded. A significant correlation between diversity of orchid and altitude was observed (r = .927, p < .05). Myrtaceae and Theaceae were the most preferred host tree families (14%) followed by Bignoniaceae (13%) and others respectively. The Study concludes that 57% of total species encountered prefers higher canopy & on a tree with rough bark, Pearson chi-square test (X2(1) = 4.7, p< .05) showed a significant difference between trunk, branches, and canopy. Many epiphytic orchids prefer dense canopy (n = 73, 47%) and living trees (99%). Future studies should be focused more on the flowering season for obtaining better information on orchid diversity in the study area.

The fragile ecosystem of greater Himalaya is home to diverse flora and fauna which are vulnerable to climate change impact. This study aimed to determine the suitable habitat of King Cobra Ophiophagus hannah (Cantor, 1836) in the current scenario and near-future scenario of the year 2040-2060 (RCP 2.6, RCP 4.5, and RCP 8.5). Geographic coordinates of its occurrence were obtained from published literature and environmental layers were obtained from worldclim.org and processed using ArcGIS and software R. The model was developed using MaxENT algorithms. The model was validated using the area under the curve (AUC) and True skill statistics (TSS), which showed that the model is very good (AUC =0.86) and (TSS=0.6). The results showed that altitude is a prime factor affecting the distribution of King Cobra in Nepal with a percent contribution of 31% followed by actual evapotranspiration 14.4% and least by Mean diurnal range (Bio2nep) 7%. The model predicted an area of 41,214 Km2 as suitable habitat for King Cobra in Nepal. The Chitwan National Park possesses a maximum suitable area (726.89 km2) followed by Chitwan-Buffer Zone (601.68 sq. Km2) and least by Sagarmatha National Park (1.73 km2) and Shey-Phoksundo-buffer zone (1.73 Km2). Our data indicate urban areas being the most suitable sites followed by open forest covers. The projection indicates contraction of 8% and 4% in a suitable area for RCP 4.5 and RCP 2.6 respectively, whereas RCP 8.5 showed expansion of 4%. Having probably suitable habitat in urban areas research proposes frequent awareness camping about conservation and protection of vulnerable King Cobra in Nepal.

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Natural habitats of Paphiopedilum fairrieanum (Lindl.) Stein was studied in the Taksha Range, Jigme Singye Wangchuck National Park, Bhutan, to determine the distribution range, population status, ecological conditions, and associated risks of the orchid. A total of 22 quadrat plots were assessed at two different locations at Rukha and Passang Omchu. The study discovered 519 individuals, mostly on steep slopes and cliffs in chirpine dominated forests with a canopy cover of less than 35% at elevations ranging from 980-1540 m above sea level. The plants were found on a limestone substrate with soil pH ranging from 7.3 to 8.0 and soil moisture content ranging from 1.4 to 2.5%, regardless of aspects and inclination. The greatest threats to the species in the study area were forest fire, browsing by wild ungulates, and anthropogenic activities.

Natural habitats of Paphiopedilum fairrieanum (Lindl.) Stein was studied in the Taksha Range, Jigme Singye Wangchuck National Park, Bhutan, to determine the distribution range, population status, ecological conditions, and associated risks of the orchid. A total of 22 quadrat plots were assessed at two different locations at Rukha and Passang Omchu. The study discovered 519 individuals, mostly on steep slopes and cliffs in chirpine dominated forests with a canopy cover of less than 35% at elevations ranging from 980-1540 m above sea level. The plants were found on a limestone substrate with soil pH ranging from 7.3 to 8.0 and soil moisture content ranging from 1.4 to 2.5%, regardless of aspects and inclination. The greatest threats to the species in the study area were forest fire, browsing by wild ungulates, and anthropogenic activities.

This study was conducted to evaluate the factors contributing to the spatial distribution of European hazelnut (Corylus avellane L.) in Bhutan and determine the suitable areas for cultivation of the crop in the near future (2040-2060) and distant future (2060-2080) under different climate scenarios of Representative Concentration Pathway (RCP)  2.6 and RCP 4.5 in Bhutan. The environmental layers were obtained from WorldClim and processed in ArcGIS and R software prior to running the model in MaxENT. The multicollinearity test showed six bioclimatic factors and three topographical factors contributing to the distribution of European hazelnut in Bhutan. The findings indicated that approximately 10,744.8 sq. km is potential for hazelnut crop cultivation in Bhutan, with Wangduephodrang District harboring the highest area of 1,227.9 sq. km (11.4%) and Gasa district the least at 130 sq. km. The primary factors affecting climate suitability of European hazelnut were elevation, contributing 87.9%, followed by Bio3 (3.7%), BIO2 (3%), and Bio1 (2.5%), and the least factors were Bio15 and aspect at 0.1% each. The model showed that the most suitable elevation for the growth of hazelnut ranges from 1500-1900 masl. The suitability area for the crop projected under the RCP 2.6 and RCP 4.5 scenarios showed a decline in the near future (2040-2060) in the southern part of the country and a slight increase in the distant future (2061-2080) in Central, Eastern, and Western Bhutan. Therefore, there is an urgent need for climate risk assessment, community-based mitigation measures, and spatial monitoring techniques on agriculture crops in Bhutan.