Pathogenic strains of Salmonella cause gastroenteritis in humans globally.This study aimed to determine the prevalence and antibiotic susceptibility pattern of Salmonellain raw milk sold in Erbil city, Kurdistanregion, Iraq. A total of 470 samples were collected from retail vendors between January and June 2019. Samples were cultured on selective media followed by serotyping and antibiotic sensitivity testing by disk diffusion assay.The results revealed that the overall prevalence of Salmonella was 7.9%. The isolates belonged to nine (9) different serotypes: S.Typhimurium 18.9%, S.Anatum 10.8%, S.Muenchen 13.6%, S.Enteritidis 10.8%, S.Senftenberg 8.1%, S. Newport 10.8%, S. Arizona 10.8%, S. Montevideo 8.1%, and S.Dublin 8.1%. The antibiotic resistance profile revealed that 67.6%, 62.2%, 56.8%,and 56.8% of isolates were resistant to levofloxacin, streptomycin, imipenem, and tetracycline respectively. This resistance among Salmonella maypose a public health threat that needs activesafety measures and response.
Background: Microbial contamination continues to be one of the leading risks to food safety. Contaminated leafy green vegetables are the primary cause of infection among children, elderly, and immunocompromised people. The purposes of this work were to isolate and identify of Salmonella spp. in fresh leafy vegetables collected from Jeddah Central Market, Jeddah district, western area, kingdom of Saudi Arabia, estimated of the number and percentage of isolated Salmonella spp and determined the antimicrobial susceptibility of the isolated Salmonella spp. Methods: Five-hundred samples were examined for the presence of Salmonella spp, by using standard microbiological and biochemical tests. Further, detection of Salmonella spp. was done by PCR with the primers targeting invA gene, a key factor for entry of Salmonella into epithelial cells. Susceptibility of the isolated Salmonella spp was done toward thirteen different antibiotics. Results: The percentage of isolation of Salmonella spp was 1.2 % (06/500). It was isolated as (0.40%, 02/500) from Basil, (0.20%, 01/500) from Spinach, Rocket, Parsley and Chards. Two isolates (2/6, 33.3%) showed positive Salmonella invA gene (244 bp). All isolated Salmonella showed resistance to Cephalexin (30 µg/disc), Metronidazole (5 µg/disc) and Methicillin (5 µg/disc).
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