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Cicer arietinum L. (chickpea) is one of most popular and cheap source of plant protein and minerals worldwide. The present study was directed to induce variations in seed characters of chickpea “Vijay”, especially with reference to increase in its protein and mineral content using gamma radiations as mutagenic agent. M2 population of Cicer arietinum L. “Vijay” after post-harvest analysis revealed that 300 Gy dose of gamma radiations induced significant variations in seed characters including seed size, surface texture and seed coat color. Total nine mutants were identified differing from control in seed coat colors and categorized into four groups on the basis of seed size as normal, small, bold and extra bold; two groups on the basis of surface texture and wrinkled or smooth surface. The seed yield of all mutants was noted as 100-seed weight, which was corresponding with seed size. The biochemical analysis of the seed mutants in the form of protein, iron and zinc content indicates that, seeds with dark colored seed coat have higher level of protein and minerals as compare to control.

SUMMARYChickpea is one of the most important leguminous food grain grown worldwide. Mutagenesis could be used as a classical way to increase genetic variability in chickpea considering its narrowing genetic base. Present study was an attempt to analyze the frequency and spectrum of chlorophyll mutations induced by gamma rays (300, 400 and 500 Grey) and ethyl methanesulphonate (0.2%, 0.3% and 0.4%) in M 2 generation of varieties of chickpea (Var-Vijay and PKV-2). Broad spectrum chlorophyll mutants were isolated from M 2 generation. The relative frequency of these mutants in both varieties was observed in order of Tigrina > Viridis > Chlorina > Xantha and Albina. The total chlorophyll mutation frequency increased with increase of dose upto certain limit and then started declining. However, the effectiveness of EMS was found to be more prominent than gamma rays especially in inducing chlorophyll mutations in Chickpea.Key Words : Chickpea, Chlorophyll mutants, Ethyl methanesulphonate, Gamma rays, Frequency How to cite this article : Bogawar, Prajwal, Koche, Deepak and Joshi-Saha, Archana (2017). Frequency and spectrum of chlorophyll mutants induced by gamma rays and EMS in two chickpea varieties (Variety-Vijay and PKV-2). Internat. J. Plant Sci.,

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