<strong>Background:</strong> For centuries, the female reproductive system has been affected by various abnormalities and diseases and hence has been the subject of interest and the basis for the gynaecological practice. The uterus being a vital reproductive and hormone-responsive organ, is subjected to a variety of physiological changes and benign and malignant disorders. <strong>Aims and Objectives:</strong> To study the indications of hysterectomy, to study gross and histopathological features of uterus and cervix in hysterectomy specimens and correlate with clinical findings. <strong>Material and Methods:</strong> This was a descriptive study of the gross and histopathological findings of uterus and cervix in 150 hysterectomy specimens received in the department. The hysterectomy specimens received were fixed in 10% formalin for 24 hours, were examined grossly and necessary sections were obtained. The tissue pieces were then processed in automated tissue processor, well labelled paraffin blocks were made. Approximately 2-3μ thickness sections were cut with the help of microtome and were stained routinely by Hematoxylin &amp; Eosin stain and special stains like PAS or other were used wherever necessary. The histopathological findings of uterus and cervix were noted and these findings were then correlated with clinical diagnosis. <strong>Results:</strong> Overall clinicopathological correlation was noted in all 150 cases. In cases of uterine fibroid it was 69.7% and 100% in case of endometrial carcinoma and cervical dysplasia. Most common pathology found was uterine leiomyomas in 48 cases and next to it was adenomyosis in 30 cases. <strong>Conclusion:</strong> It can be concluded that clinico-pathological correlation in case of endometrial cancer and uterine fibroid is excellent, but in case of DUB and prolapse uterus it varies. This signifies the importance of clinico-pathological correlation in all cases of hysterectomy to improve the clinical outcome and post-operative management.

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A sensitive, stability-indicating reversed-phase high-performance liquid chromatographic method was developed for the determination of eszopiclone and related impurities in tablet dosage form. The chromatographic separation was achieved on an Inertsil C18 column (250 × 4.6 mm, 5 µm), using a mobile phase consisting of 0.05M monobasic sodium phosphate buffer containing 0.8% sodium lauryl sulfate (pH 3.5) and acetonitrile in the ratio of 60:40 (v/v), at a flow rate of 1.5 mL/min and temperature of 40°C. Quantification was achieved with photodiode array detection at 303 nm. The described method showed excellent linearity over a range of limits of quantification to 4.8 µg/mL (150% of specification limit; i.e., 3.2 µg/mL). The drug product was subjected to the stress conditions of oxidative, acid, base, thermal and photolytic degradation. Eszopiclone degradation was observed in acid hydrolysis, base hydrolysis and peroxide stress conditions. Eszopiclone was stable in thermal and photolytic degradation conditions. The developed method is simple, selective and accurate for the quantification of impurities and degradation products of eszopiclone in tablet dosage form.

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Background:Hypertension shows circadian rhythm that there is a rise in pressure from the time of waking or before (about 4 to 8 a.m.), in most people. Conventional drug delivery system of captopril is inappropriate for the delivery of drug, as they cannot be administered just before the symptoms are worsened, because during this time the patients are asleep, bedtime dosing of captopril will not provide a therapeutic plasma drug concentration at the early hours of morning because of poor pharmacokinetic profile and shorter half-life of 1.9 hours. Thus, this study attempts to design and evaluate a chronomodulated pulsatile drug delivery system of captopril which was aimed to release the drug after a lag time of 6 hours.Materials and Methods:Present delivery system was prepared by rupturable coating method. The core containing captopril as a bioactive compound were prepared by direct compression method and then coated sequentially with an inner swelling layer containing hydrocolloid HPMC E5 and an outer rupturable layer consisted of Eudragit RL/RS (1 : 1). Total 12 formulations with different levels of inner swelling layer and outer polymeric layer were prepared and subjected to various processing and formulative parameters like the effect of core composition, level of swelling layer, and rupturable coating on lag time was investigated. In vitro drug release and rupture tests were performed using United States Pharmacopoeia paddle method at 50 rpm in 0.1N HCl and phosphate buffer of pH 6.8.Results:The results showed that as the amount of inner swelling layer increases, the lag time decreases and as the Eudragit coating level increases, the lag time increases and percent water uptake of time-dependent pulsatile release system decreases. The presence of an osmotic agent and effervescent agent helped in shortening of lag time.Conclusion:The system was found to be satisfactory in terms of release of the drug after the lag time of 6 hours.

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