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PurposeWe examined expression profiles of 16 micro RNAs (miRNAs) in triple negative breast cancers to identify their potential as biomarkers for lymph node metastasis.MethodsThe expression profiles of miR-9, miR-21, miR-30a, miR-30d, miR-31, miR-34a, miR-34c, miR-100, miR-122, miR-125b, miR-146a, miR-146b, miR-155, miR-181a, miR-200c, and miR-205 were examined by using real-time quantitative reverse transcription polymerase chain reaction in tumor samples and corresponding benign breast tissues. Their associations with histopathological features and prognostic parameters were assessed.ResultsWhen compared with the expression in benign breast tissues, seven of the miRNAs (miR-31, miR-205, miR-34a, miR-146a, miR-125b, miR-34c, and miR-181a) were downregulated more than 1.5-fold in tumor tissues, whereas, only miR-21 was found to be upregulated more than 1.5-fold in tumor tissues. Although miR-200c levels were decreased only 1.12-fold in tumor tissues, the reduced expressions of miR-200c and miR-205 were significantly associated with lymph node metastasis (p=0.021 and p=0.016, respectively).ConclusionOur results demonstrate that miR-205 and miR-200c expression levels may be useful in predicting lymph node metastasis in triple negative breast cancer patients.

The primary purpose of our work is to show that the vidian canal can be determined on CT imaging. The secondary goal is to establish the position and configuration of the vidian canal visualization. The CT imaging of vidian canals of 150 patients were examined in axial and coronal sections in 3 mm thickness made at 3 mm intervals. The varying course of the canal inside the bone, bone structure and diameter of the canal were investigated. In all cases, the canal was seen, and in 4 of the cases (1.3%) the canal was separated into two parts by a septum on its initial portion. In 36% of the cases it was embedded in the sphenoid corpus, partially protruded in 54% of the cases, and was connected to the bone with a stalk inside the sinus in 10% of the cases. The bony structure of the canal showed continuation in 68%, with 32% showing dehisans. The incidence of dehisans was 77% in the stalked cases and 45% in the protruded cases. It was determined that it is possible to evaluate the position and configuration of the vidian canal with CT imaging. This may be useful for diagnosing vidian nerve pathology and performing surgical intervention such as vidian neuroectomy and sinus surgery.

The aim of this study is to investigate the effects of different fixatives on DNA, and to evaluate the fixation options for molecular studies including polymerase chain reaction (PCR) and fluorescence in-situ hybridization (FISH). Three normal-looking colonic segments from surgical resections were used for tissue sampling. The full thickness of the colonic tissues (3 mm diameter) was sampled. Tissues were fixed in 70% ethanol, 10% neutral-buffered formalin, Hollande, B5, Bouin, and Zenker solutions for 1, 2, 5, 12, 24, and 48 hours, and processed and embedded in paraffin in a standard protocol. Quantitative measurements of the extracted DNA were carried out. DNA quality was tested by PCR for the human beta globin gene. Tissue sections were also tested for the availability of FISH, by using a Her-2/neu protocol. All fixation alternatives were found to be reasonable sources of DNA for molecular studies, and they enabled the successful PCR amplification of a housekeeping gene. DNA yields were predominantly over 1000 bp in 70% ethanol and 24-hour 10% neutral-buffered formalin fixations. As for B5 and Hollande, the DNA molecules obtained were almost all smaller than 100 bp. All tissues fixed in formalin, ethanol, and Hollande were found suitable for Her-2/neu visualization after standard FISH applications, but tissues fixed in Zenker, B5, and Bouin were not found suitable.

A 67-year-old female with type 2 diabetes mellitus developed nephrotic syndrome with in one week of receiving the first dose of SARS-CoV-2 CoronaVac vaccine. A kidney biopsy was consistent with minimal change nephrotic syndrome and treatment was symptomatic with antiproteinurik therapy and improvement in proteinuria. Edema returned within one week of the second dose of CoronaVac. In this occasion acute kidney injury and massive proteinuria were noted. In kidney biopsy, glomeruli were normal, but tubulointerstitial inflammation consistent with acute tubulointerstitial nephritis were noted. Pulse followed by oral steroids were followed by recovery of kidney function. Proteinuria decreased after initiation of cyclosporine A.

Neurofibromas arise from elements in the peripheral nervous system and are rarely detected in the breast. Neurofibromatosis is a phakomatosis that displays a wide spectrum of clinical expression with neurocutaneous abnormalities and involvement of multiple organ systems. Neurofibromas are more common in neurofibromatosis Type 1. They can show variable amounts of myxoid change, which affect their radiological findings. In this paper, we present a patient with neurofibromatosis Type 1 with mammographical, sonographical and MRI findings of myxoid neurofibromas in her left breast.

HSP 70 levels increase in the myocardium of rats in diabetes mellitus as a protective mechanism. Levels of HSP 70 may further be increased with parenteral administration of glutamine. Efficacy of glutamine is more pronounced in left heart structures.

Abstract:The current use of doxorubicin is regarded as an absolute contraindication for hyperbaric oxygen (HBO 2 ) therapy because of the increased risk of cardiotoxicity. The aim of this study was to investigate whether additional exposure to HBO 2 during the course of doxorubicin treatment would further increase the cardiotoxicity of doxorubicin in rats. Female Wistar rats were treated with either HBO 2 (n = 10) or doxorubicin (n = 8) or a combination of both treatments (n = 10) for 4 consecutive weeks and followed up for an additional 4 weeks. Cardiomyopathy was evaluated using two-dimensional and M-mode echocardiography at baseline, at the fourth, sixth and eighth weeks, and by histopathological investigation of the rat hearts at the eighth week. Doxorubicin treatment significantly reduced ejection fraction and fractional shortening (P < 0.001) and caused severe histopathological injury (P < 0.05) indicating development of cardiotoxicity. Although the combination of doxorubicin and HBO 2 also markedly reduced ejection fraction and fractional shortening (P < 0.001), this reduction was significantly less than that of doxorubicin treatment (P < 0.05). HBO 2 therapy also attenuated doxorubicin-induced histopathological changes in rat hearts (P < 0.05). HBO 2 alone did not alter echocardiographic parameters or histopathological findings (P > 0.05). In conclusion, HBO 2 therapy does not potentiate doxorubicin-induced cardiotoxicity in rats. Cardioprotection conferred by HBO 2 against doxorubicin warrants further investigation.