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This work presents the effects of changes in temperature (4 °C, 25 °C, and 40 °C), pH (6, 7, and 9), and polymer molecular weight (1000 and 8000) on the equilibrium phase behavior of two-phase PEG + potassium phosphate + water systems. As the temperature and pH were increased, the binodal curve shifted to lower polyethylene and phosphate concentrations, resulting in a higher difference in polymer and salt concentrations between the top and bottom phases. This corresponds to an increase in the region of two-phase coexistence.

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