e21128 Background: Radiotherapy alone or in combination with systemic therapies, is a major tool for local tumor control. Although organ-targeting techniques have greatly been improved over the past years, radiation enteropathy is a dose-limiting factor for effective radiotherapy. The out-of-field effects on the intestine, caused by radiation treatment of a parenchymatous organ, have not previously been studied. Methods: A single dose of 25 Gray was administered percutaneously to the liver of male Wistar rats after a planning CT-scan. Sham-irradiated animals served as controls. At 1, 6, 24, 96 hours, 1.5 and 3 months the duodenum, jejunum, ileum and distal colon were removed, washed and deep-frozen or prepared for paraffin staining. Results: All animals survived the treatment. Epithelial cell damage occurred in all small-intestinal segments. However, prolonged denudation of the villi together with destruction of the crypt lining was only observed in the ileum, which only received scattered radiation. Whilst the intestinal lining in the duodenum and jejunum was restored at the end of the study, in the ileum, tissue regeneration was deficient. In the colon, changes were minor. Immunhistochemical staining showed radiation mucositis with granulocyte (MP0+) infiltration from 1 to 24 hours in the duodenum and jejunum, when ED1+ macrophages, CD3+ T-lymphocytes, and CD34+ hematopoietic precursor cells were recruited, accompanied by an increase in the chemokines MCP-1, MIP-1α, MIP3α and Il-8. In the ileum, early granulocyte infiltration was delayed but continuous. Recruitment of macrophages and lymphocytes was deficient and induction of chemokines as of the adhesion molecules PECAM-1, ICAM-1 was lacking. Conclusions: Post-irradiation damage to the ileum was delayed and followed by an altered repair process with structural changes of the villi. The observed changes might result from a higher sensitivity to oxidative stress mechanisms with subsequent damage of the regenerative capacity of the crypt-villus axis, accompanied by a sustained “inflammatory response” and vascular damage with a lack of regeneratory cell recruitment.
APOPTOSE 1.2.1 MORPHOLOGIE DER APOPTOSE 1.2.2 APOPTOSE VERSUS NEKROSE 1.2.3 CASPASEN: DIE SCHLÜSSELENZYME DER APOPTOSE 1.2.4 SIGNALTRANSDUKTIONSWEGE DER APOPTOSE 1.3 ZYTOKINE: DIE REGULATION DER INTESTINALEN ZELLHOMÖOSTASE 1.4 ZIELSETZUNG 2 MATERIAL UND METHODEN 2.1 VERSUCHSAUFBAU 2.2 RNA-NACHWEIS 2.2.1 RNA-EXTRAKTION AUS GEWEBE MIT HILFE DER ULTRAZENTRIFUGE 2.2.2 RNA-KONZENTRATIONSBESTIMMUNG 2.2.3 REVERSE TRANSKRIPTION 2.2.4 REAL-TIME-PCR 2.2.5 STATISTISCHE ANALYSE DER PCR-DATEN 2.3 GELELEKTROPHORESE ZUR DETEKTION VON APOPTOTISCHER DNA 2.4 WESTERN BLOT: NACHWEIS VON PROTEINEN 2.4.1 PROTEINISOLATION 2.4.2 BESTIMMUNG DER PROTEINKONZENTRATION VON EINZELPROBEN 2.4.3 HERSTELLUNG DER POLYACRYLAMIDGELE 2.4.4 GELELEKTROPHORETISCHE TRENNUNG DES PROTEINGEMISCHES 2.4.5 GEL-BLOT: SEMI-DRY-VERFAHREN 2.4.6 PROTEINBANDENNACHWEIS MIT PONCEAU-ROT 2.4.7 BLOCKING 2.4.8 ANTIKÖRPER 2.4.9 ENTWICKLUNGSPROZESS 2.4.10 STRIPPEN DER MEMBRANEN 2.5 HISTOLOGIE 2.5.1 HÄMATOXYLIN-EOSIN-FÄRBUNG 2.5.2 MASSON-GOLDNER-TRICHROMFÄRBUNG 2.5.3 TUNEL-FÄRBUNG 2.5.4 IMMUNHISTOCHEMIE 2.6 PUFFER UND VERWENDETE LÖSUNGEN 3 ERGEBNISSE 3.1 DAS VERSUCHSTIER: RATTE 3.2 HISTOLOGISCHE VERÄNDERUNG IN FOLGE VON BESTRAHLUNG 3.2.1 H.E.-FÄRBUNG: DIE ÜBERSICHTSFÄRBUNG 3.2.2 MASSON-GOLDNER-TRICHROMFÄRBUNG IV 3.2.3 ED1 ALS MARKER FÜR CD68-POSITIVE MAKROPHAGEN 3.2.4 CD3 ALS MARKER FÜR T-LYMPHOZYTEN 3.2.5 CD34-FÄRBUNG 3.2.6 CASPASE-3-FÄRBUNG 3.2.7 TUNEL-FÄRBUNG 3.3 ERGEBNISSE DER RNA-ANALYSE 3.3.1 PROINFLAMMATORISCHE ZYTOKINE 3.3.2 IP-10: DAS ETWAS ANDERE ZYTOKIN 3.3.3 FIBROSIERUNG IM DARM INITIIERT DURCH TGF- 3.3.4 DIE EXPRESSION VON CHEMOKINEN UND ADHÄSIONSMOLEKÜLEN 3.3.5 DIE EXPRESSION VON PRO-UND ANTIAPOPTOTISCHEN FAKTOREN 3.3.6 DIE EXPRESSION VON ENTZÜNDUNGSMEDIATOREN UND IMMUNZELLEN 3.3.7 DIE ANGIOGENESE BEEINFLUSSENDEN FAKTOREN: VEGF, VEGF-R UND SMA 3.4 ERGEBNISSE DER WESTERN BLOTS 3.4.1 -AKTIN, DAS HOUSE-KEEPING GEN 3.4.2 CASPASE 8 3.4.3 CASPASE 3 3.4.4 DAS PROAPOPTOTISCHE BAX 3.4.5 DER GEGENSPIELER DER APOPTOSE: BCL-2 3.5 ANALYSE VON GENOMISCHER DNA MIT HILFE EINER GEL-ELEKTROPHORESE 3.6 ZUSAMMENFASSUNG DER ERGEBNISSE 4 DISKUSSION 4.1 BESTRAHLUNGSMODELLE 4.1.1 WAHL DER DARMABSCHNITTE UND ZEITPUNKTE NACH BESTRAHLUNG 4.1.2 METHODISCHE DURCHFÜHRUNG 4.2 PATHOLOGISCHE VERÄNDERUNGEN AM DARM IN FOLGE VON BESTRAHLUNG 4.2.1 APOPTOSE: DIREKTE ANTWORT AUF BESTRAHLUNG 4.2.2 AKUTE UND CHRONISCHE STRAHLENENTERITIS 4.2.3 CHEMOTAXIS UND MIGRATION VON IMMUNZELLEN 4.2.4 ENDOTHELIALE DYSFUNKTION UND HYPOXIE 4.2.5 STRAHLENINDUZIERTE FIBROSIERUNG AM DARM: LANGZEITSCHÄDEN 4.3 PARALLELEN ZU ANDEREN PATHOLOGISCHEN DARMVERÄNDERUNGEN 4.4 MÖGLICHE THERAPIEANSÄTZE UND KLINISCHE RELEVANZ 4.5 AUSBLICKE
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