SummaryWe hypothesized that plant exudates could either gel or disperse soil depending on their chemical characteristics. Barley (Hordeum vulgare L. cv. Optic) and maize (Zea mays L. cv. Freya) root exudates were collected using an aerated hydroponic method and compared with chia (Salvia hispanica L.) seed exudate, a commonly used root exudate analogue. Sandy loam soil was passed through a 500‐μm mesh and treated with each exudate at a concentration of 4.6 mg exudate g−1 dry soil. Two sets of soil samples were prepared. One set of treated soil samples was maintained at 4°C to suppress microbial processes. To characterize the effect of decomposition, the second set of samples was incubated at 16°C for 2 weeks at −30 kPa matric potential. Gas chromatography–mass spectrometry (GC–MS) analysis of the exudates showed that barley had the largest organic acid content and chia the largest content of sugars (polysaccharide‐derived or free), and maize was in between barley and chia. Yield stress of amended soil samples was measured by an oscillatory strain sweep test with a cone plate rheometer. When microbial decomposition was suppressed at 4°C, yield stress increased 20‐fold for chia seed exudate and twofold for maize root exudate compared with the control, whereas for barley root exudate decreased to half. The yield stress after 2 weeks of incubation compared with soil with suppressed microbial decomposition increased by 85% for barley root exudate, but for chia and maize it decreased by 87 and 54%, respectively. Barley root exudation might therefore disperse soil and this could facilitate nutrient release. The maize root and chia seed exudates gelled soil, which could create a more stable soil structure around roots or seeds.Highlights Rheological measurements quantified physical behaviour of plant exudates and effect on soil stabilization.Barley root exudates dispersed soil, which could release nutrients and carbon.Maize root and chia seed exudates had a stabilizing effect on soil.Physical engineering of soil in contact with plant roots depends on the nature and origin of exudates.

Summary In this paper, we provide direct evidence of the importance of root hairs on pore structure development at the root–soil interface during the early stage of crop establishment.This was achieved by use of high‐resolution (c. 5 μm) synchrotron radiation computed tomography (SRCT) to visualise both the structure of root hairs and the soil pore structure in plant–soil microcosms. Two contrasting genotypes of barley (Hordeum vulgare), with and without root hairs, were grown for 8 d in microcosms packed with sandy loam soil at 1.2 g cm−3 dry bulk density. Root hairs were visualised within air‐filled pore spaces, but not in the fine‐textured soil regions.We found that the genotype with root hairs significantly altered the porosity and connectivity of the detectable pore space (> 5 μm) in the rhizosphere, as compared with the no‐hair mutants. Both genotypes showed decreasing pore space between 0.8 and 0.1 mm from the root surface. Interestingly the root‐hair‐bearing genotype had a significantly greater soil pore volume‐fraction at the root–soil interface.Effects of pore structure on diffusion and permeability were estimated to be functionally insignificant under saturated conditions when simulated using image‐based modelling.

Summary Soil adjacent to roots has distinct structural and physical properties from bulk soil, affecting water and solute acquisition by plants. Detailed knowledge on how root activity and traits such as root hairs affect the three‐dimensional pore structure at a fine scale is scarce and often contradictory. Roots of hairless barley (Hordeum vulgare L. cv Optic) mutant (NRH) and its wildtype (WT) parent were grown in tubes of sieved (<250 μm) sandy loam soil under two different water regimes. The tubes were scanned by synchrotron‐based X‐ray computed tomography to visualise pore structure at the soil–root interface. Pore volume fraction and pore size distribution were analysed vs distance within 1 mm of the root surface. Less dense packing of particles at the root surface was hypothesised to cause the observed increased pore volume fraction immediately next to the epidermis. The pore size distribution was narrower due to a decreased fraction of larger pores. There were no statistically significant differences in pore structure between genotypes or moisture conditions. A model is proposed that describes the variation in porosity near roots taking into account soil compaction and the surface effect at the root surface.

Using rhizosphere-scale physical measurements, we tested the hypothesis that plant exudates gel together soil particles and, on drying, enhance soil water repellency. Barley (Hordeum vulgare L. cv. Optic) and maize (Zea mays L. cv. Freya) root exudates were compared with chia (Salvia hispanica L.) seed exudate, a commonly used root exudate analog. Sandy loam and clay loam soils were treated with root exudates at 0.46 and 4.6 mg exudate g −1 dry soil and chia seed exudate at 0.046, 0.46, 0.92, 2.3 and 4.6 mg exudate g −1 dry soil. Soil hardness and modulus of elasticity were measured at −10 kPa matric potential using a 3-mm-diameter spherical indenter. The water sorptivity and repellency index of air-dry soil were measured using a miniaturized infiltrometer device with a 1-mm tip radius. Soil hardness increased by 28% for barley root exudate, 62% for maize root exudate, and 86% for chia seed exudate at 4.6 mg g −1 concentration in the sandy loam soil. For the clay loam soil, root exudates did not affect soil hardness, whereas chia seed exudate increased soil hardness by 48% at 4.6 mg g −1 concentration. Soil water repellency increased by 48% for chia seed exudate and 23% for maize root exudate but not for barley root exudate at 4.6 mg g −1 concentration in the sandy loam soil. For the clay loam soil, chia seed exudate increased water repellency by 45%, whereas root exudates did not affect water repellency at 4.6 mg g −1 concentration. Water sorptivity and repellency were both correlated with hardness, presumably due to the combined influence of exudates on the hydrological and mechanical properties of the soils.Abbreviations: DW, distilled water.Exudates produced by plant roots and microbes continually modify plant-soil interactions such as root penetration, soil aggregate formation, microbial dynamics, and water and nutrient fluxes from soil to roots (Carminati et al., 2016;Oleghe et al., 2017;Hinsinger et al., 2009). It has been well documented in a number of species such as sorghum [Sorghum bicolor (L.) Moench], wheat (Triticum aestivum L.), and rice (Oryza sativa L.) that root exudation decreases with the age of the plant and increases with soil stress such as compaction, drought, and limited nutrient supply (Neumann et al., 2014;Aulakh et al., 2001;Brady and Weil, 1999). Plant exudates are generally viscoelastic gels consisting of an array of compounds such as large molecular weight polysaccharides (with both free sugars and polymerized arabinose, fructose, glucose, maltose, xylose, etc.), organic acids (acetic, gluconic, succinic, valeric acids, etc.), amino acids (alanine, glycine, lysine, valine, etc.), fatty acids, and sugar alcohols Aulakh et al., 2001).Plant exudates can have a large influence on soil mechanical stability through resistance to disruption by mechanical and hydraulic stresses that depend on exudate chemical characteristics. The anions of organic acids present in root exudates may be adsorbed by soil mineral particles, thereby increasing the net negative charge of clays that would cause p...

The extent (determined by the repellency indices RI and RIc) and persistence (determined by the water drop penetration time, WDPT) of soil water repellency (SWR) induced by pines were assessed in vastly different geographic regions. The actual SWR characteristics were estimated in situ in clay loam soil at Ciavolo, Italy (CiF), sandy soil at Culbin, United Kingdom (CuF), silty clay soil at Javea, Spain (JaF), and sandy soil at Sekule, Slovakia (SeF). For Culbin soil, the potential SWR characteristics were also determined after oven-drying at 60°C (CuD). For two of the three pine species considered, strong (Pinus pinaster at CiF) and severe (Pinus sylvestris at CuD and SeF) SWR conditions were observed. Pinus halepensis trees induced slight SWR at JaF site. RI and RIc increased in the order: JaF < CuF < CiF < CuD < SeF, reflecting nearly the same order of WDPT increase. A lognormal distribution fitted well to histograms of RIc data from CuF and JaF, whereas CiF, CuD and SeF had multimodal distributions. RI correlated closely with WDPT, which was used to develop a classification of RI that showed a robust statistical agreement with WDPT classification according to three different versions of Kappa coefficient.

Background and aimsAlternate wetting and drying (AWD) saves water in paddy rice production but could influence soil physical conditions and root growth. This study investigated the interaction between contrasting rice genotypes, soil structure and mechanical impedance influenced by hydraulic stresses typical of AWD.MethodsContrasting rice genotypes, IR64 and deeper-rooting Black Gora were grown in various soil conditions for 2 weeks. For the AWD treatments the soil was either maintained in a puddled state, equilibrated to −5 kPa (WET), or dried to −50 kPa and then rewetted at the water potential of −5 kPa (DRY-WET). There was an additional manipulated macropore structure treatment, i.e. the soil was broken into aggregates, packed into cores and equilibrated to −5 kPa (REPACKED). A flooded treatment (puddled soil remained flooded until harvest) was set as a control (FLOODED). Soil bulk density, penetration resistance and X-ray Computed Tomography (CT) derived macropore structure were measured. Total root length, root surface area, root volume, average diameter, and tip number were determined by WinRhizo.ResultsAWD induced formation of macropores and slightly increased soil mechanical impedance. The total root length of the AWD and REPACKED treatments were 1.7–2.2 and 3.5–4.2 times greater than that of the FLOODED treatment. There was no significant difference between WET and DRY-WET treatments. The differences between genotypes were minimal.ConclusionsAWD influenced soil physical properties and some root characteristics of rice seedlings, but drying soil initially to −50 kPa versus −5 kPa had no impact. Macropores formed intentionally from repacking caused a large change in root characteristics.

Biological exudates, such as plant mucilage, can greatly stabilize soils, but as the mechanical and hydrological drivers depend much on soil particle size composition, eroding and depositional areas of a slope may respond differently. Soils from an eroded midslope and a depositional footslope in an arable farm were amended with chia (Salvia hispanica) seed mucilage at concentrations of 0 g C kg−1, 0.46 g C kg−1 and 2.3 g C kg−1 mucilage, formed into cores, and then imparted with wetting and drying (WD) cycles. Mucilage increased the stability of these inherently stable soils from 80% to >98% water‐stable macroaggregates at 0 WD cycles regardless of slope position. Aggregate stability was maintained after 5 WD cycles by mucilage, whereas the stability of unamended soil dropped by 66.7% in the footslope and 30.1% in the midslope compared with 0 WD. The underlying physical stability properties were measured by tensile strength and penetration resistance for mechanical properties, water sorptivity and repellency for hydrological properties, and micro‐, meso‐, macro‐ and total porosity for structural properties. Almost every soil physical property measured changed less with WD cycles if mucilage was present. Compared to unamended soil, 2.3 g C kg−1 mucilage amendment decreased water sorptivity from 0.289 mm s−1/2 to 0.122 mm s−1/2 in the midslope and 0.230 mm s−1/2 to 0.182 mm s−1/2 in the footslope after 5 WD cycles. Aggregate stability, total porosity and water sorptivity were correlated. In the midslope, hydrology and penetration resistance were affected the most, which was likely to be driven by mucilage deposition in the macropores of this more coarsely textured soil. In the footslope, the greater impact of mucilage on tensile strength was likely to be driven by buffering of macroporosity formation by WD cycles in this finer‐textured soil. Highlights We explored how slope position interacts with plant mucilage to drive soil physical stability. Changes in soil physical stability by plant mucilage have rarely been considered with slope position. Interactions between mucilage and soil particles caused greater physical stability in the midslope than footslope. Mucilage stabilized soil by easing changes in pore structure, DOC redistribution and water repellency, with particle bonding less important.

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