The selective serotonin re-uptake inhibitor (SSRI) fluoxetine is widely used in the treatment of depression in children and fertile women, but its effect on developing tissues has been sparsely investigated. The aim of this study was to investigate if enamel organs and ameloblast-derived cells express serotonin receptors that are affected by peripherally circulating serotonin or fluoxetine. Using RT-PCR and western blot analysis we found that enamel organs from 3-d-old mice and ameloblast-like cells (LS8 cells) express functional serotonin receptors, the rate-limiting enzyme in serotonin synthesis (Thp1), as well as the serotonin transporter (5HTT), indicating that enamel organs and ameloblasts are able to respond to serotonin and regulate serotonin availability. Fluoxetine and serotonin enhanced the alkaline phosphatase activity in the cell culture medium from cultured LS8 cells, whereas the expression of enamelin (Enam), amelogenin (Amel), and matrix metalloproteinase-20 (MMP-20) were all significantly down-regulated. The secretion of vascular endothelial growth factor (VEGF), monocyte chemotactic protein 1 (MCP-1), and interferon-inducible protein 10 (IP-10) was also reduced compared with controls. In conclusion, enamel organs and ameloblast-like cells express functional serotonin receptors. Reduced transcription of enamel proteins and secretion of vascular factors may indicate possible adverse effects of fluoxetine on amelogenesis.
Reports indicate that oil/water mouthrinses with an aqueous phase containing an antibacterial agent, reduce the amount of volatile bacterial products in expiration air compared with aqueous mouthrinses. These systems have not, however, been tested concerning antiplaque activity. The aim of the present study was to examine the plaque-inhibiting effect of a mouthrinse with an aqueous phase containing 0.2% chlorhexidine (CHX) and an oily phase (soya oil) containing 0.3% triclosan. A test panel rinsed with the mouthrinses twice daily for 4 d. The mouthrinse containing CHX and triclosan in two phases was significantly better than the negative control (water). However, it was not as effective as the rinse consisting of an aqueous phase with chlorhexidine combined with an oily phase without triclosan. A two-phase mouthrinse with soya oil containing 0.3% triclosan was not superior to soya oil alone, and the combination of CHX and triclosan in a two-phase rinse was not as effective as 0.1% CHX alone in water. No beneficial effect on plaque inhibition could thus be found by using a two-phase system with two different antibacterial agents (one water soluble and one lipid soluble). Soya oil without triclosan rendered higher plaque inhibition than the control, presumably due to formation of a hydrophobic layer on the tooth surfaces.
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