The content of two groups of compounds with biological activity (non-hallucinogenic indole compounds and free phenolic acids) were analyzed in extracts of fruiting bodies of four species of Phellinus: P. igniarius, P. pini, P. pomaceus and P. robustus. The presence of indole compounds in methanolic extracts was analyzed by high-performance liquid chromatography and thin-layer chromatography coupled with densitometric detection. Three metabolites (serotonin, tryptamine, and L-tryptophan) were identified. The contents of individual indole compounds ranged from 1.70 (tryptamine in P. robustus) to 8.32 mg  100 g-1 dry weight (L-tryptophan in P. robustus). Four free phenolic acids were detected in methanolic extracts by the HPLC method. The total content ranged from 9.9 mg  100 g-1 DW (P. igniarius) to 32.5 mg  100 g-1 DW (P. robustus).

In the presented work, raw materials (fruits and leaves) and in vitro biomass of a highly productive Schisandra chinensis Sadova No. 1 cultivar (SchS) were evaluated for the production of therapeutically useful schisandra lignans (SL). In vitro cultures of SchS were initiated, followed by extensive optimization studies focused on maximizing secondary metabolite production, with the aim of establishing a sustainable source of SL. Different cultivation systems (agar, agitated, bioreactor), experiment times (10, 20, 30, 40, 50 and 60 days) and plant growth regulators (6-benzyladenine—BA and 1-naphthaleneacetic acid—NAA, from 0 to 3 mg/l) in Murashige-Skoog (MS) medium were tested. Moreover, an elicitation procedure was applied to bioreactor-grown microshoots in order to increase SL production. Validated HPLC-DAD protocol enabled to detect fourteen SL in the extracts from in vitro and in vivo materials. The main compounds in the in vitro cultures were as follows: schisandrin (max. 176.3 mg/100 g DW), angeloylgomisin Q (max. 85.1 mg/100 g DW), gomisin A (max. 71.4 mg/100 g DW) and angeloylgomisin H (max. 67.0 mg/100 g DW). The highest total SL content (490.3 mg/100 g DW) was obtained in extracts from the biomass of agar cultures cultivated for 30 days on the MS medium variant containing 3 mg/l BA and 1 mg/l NAA. This amount was 1.32 times lower than in fruit extracts (646.0 mg/100 g DW) and 2.04 times higher than in leaf extracts (240.7 mg/100 g DW). The study demonstrated that SchS is a rich source of SL, thus proving its value for medical, cosmetic and food industry.Electronic supplementary materialThe online version of this article (10.1007/s00253-018-8981-x) contains supplementary material, which is available to authorized users.

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The objective of this study was to obtain the cultures of under controlled conditions and investigate the release of indole compounds and zinc from the mycelium of to artificial digestive juices under conditions similar to those in the human gastrointestinal tract. Biomass was obtained from cultures grown using both only the Oddoux medium as well as the same medium with added zinc hydroaspartate and zinc sulfate. The release of 5-hydroxy-L-tryptophan, L-tryptophan, and serotonin from the biomass extracts to the artificial digestive juices was determined. Differential pulse anodic stripping voltammetry was used to demonstrate that zinc is released from each of the extracted materials. The total amount of zinc in the materials under study was estimated to be between 7.12 and 44.15 mg/100 g dry weight. It was demonstrated that cultures of grown using appropriately selected media may supplement zinc and indole compounds.

Summary. The analysis demonstrated that biomass of Agaricus bisporus, Boletus badius,and Cantharellus cibarius contains non-hallucinogenic indole compounds. Addition of L-tryptophan to the in vitro cultures raised the total content of indole compounds. L-Tryptophan became metabolized, causing an increase of the concentration of some indole compounds. The compounds found in the tested biomass from in vitro culture on Oddoux medium without and with addition of L-tryptophan were L-tryptophan, 5-hydroxy-L-tryptophan, serotonin, melatonin, tryptamine, and 5-methyltryptamine (ranged from 4.28 to 132.51 mg/100 g dry weight). L-Tryptophan is an amino acid exogenous to the human body, and therefore, it must be supplied to the body with food. The highest amount of 5-hydroxy-L-tryptophan was found in the extracts from biomass of B. badius cultured on medium with addition of L-tryptophan (132.51 mg/100 g dry weight). Also, in this case, the highest total content of examined indole compounds (168.00 mg/100 g dry weight) was determined. Melatonin was found only in biomass of A. bisporus cultured on medium with addition of L-tryptophan but in smaller amount (4.28 mg/100 g dry weight).

Azole antifungal agents are widely used as active ingredients in antifungal pharmaceuticals and agricultural fungicides. An increase in the use of azole antifungals has resulted in an increase in the concentration of these compounds in wastewater and surface water, with potential implications for agriculture. In the present study, bifonazole (BIF) and clotrimazole (CTZ) were selected for investigation because of their widespread use in topical formulations and persistence in the environment. The mycoremediation capacity of BIF and CTZ by mycelia of Lentinula edodes in in vitro culture was evaluated. The main aim of this study was to identify the presumable biodegradation products of the investigated active pharmaceutical substances using the LC/MS/MS method. For this purpose, the media were enriched with the following active pharmaceutical ingredients selected for this study: BIF powder, CTZ powder, and BIF cream, each of them at the same concentration of 0.1 mg/mL. Subsequently, thin-layer chromatography coupled with densitometry was used to evaluate the content of BIF and CTZ in mycelium from in vitro cultures of L. edodes. The degradation process was found to affect primarily the imidazole moiety of both investigated compounds. In addition, the amounts of undegraded investigated compounds were found to be 4.98, 9.26, and 4.56 mg/g dry weight for BIF powder, CTZ powder, and BIF cream, respectively. Therefore, the findings of this study revealed that L. edodes could be considered for remediation of pollution caused by azole antifungal agents.

A thin-layer chromatography (TLC)-densitometry method has been developed to identify and quantify haloperidol, amitriptyline, sulpiride, promazine, fluphenazine, doxepin, diazepam, trifluoperazine, clonazepam, and chlorpromazine in selected psychotropic drugs. Separation was performed on precoated silica gel 60 F254 TLC plates. Chromatograms were developed in various mobile phases, and 8 of 30 tested phases were selected based on spot location and developing time. The identification and quantification were carried out based on ultraviolet densitometric measurements at chosen wavelengths. In addition to retention coefficients, the absorption spectra recorded directly from chromatograms were also used in qualitative analysis. Under established experimental conditions, high sensitivity of the method was achieved. The limit of detection ranged from 0.009 to 0.260 μg, depending on the wavelength selected for measuring. A satisfactory recovery, ranging from 92.99 to 104.70%, was achieved for individual constituents.