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Fuel Injection is a significant factor when biodiesel-diesel blends are fired in diesel engines as they have very diverse property when related to diesel. The current work describes unusual experimental study of CI engine fuelled with Waste cooking methyl ester with copper oxide nano additives at varying fuel injection pressures. The Copper oxide nano additives were manufactured using homogenous addition method and were subjected to studies such as XRD and SEM characterization. These synthesized nanoparticles were later added in waste cooking oil biodiesel blend (WCO20) with levels of 10 ppm, 20 ppm, 30 ppm, and 40ppm. The results were noted from a DI-CI VCR engine coupled with an eddy current dynamometer. The addition of CuO particles reduces the ignition delay of WCO20 and raises the thermal efficiency of the engine by an average of 3.9% and limits HC emission by 15.6%.