Surface topography and the enzyme activity of soft bioactive probes can be investigated simultaneously with a new technique. More about this method, a combination of atomic force and scanning electrochemical microscopy, can be found in the following Communication by C. Kranz et al.
Integrated submicroelectrodes for combined AFM-SECM measurements are characterized with numerical simulations using the boundary element method. SECM approach curves and SECM images are calculated and analyzed for a model substrate containing pronounced topographical and electrochemical features. The theoretically calculated image has been compared to the experimental data and shows excellent quantitative agreement. Hence, the applicability of integrated AFM-SECM electrodes for combined electrochemical and topographical imaging and a profound theoretical description including quantification of the obtained results are demonstrated.
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