weeks of gestation) from healthy subjects undergoing elective cesarean section. Syncitiotrophoblasts (STB) were purified and treated for 24 hours with physiologically relevant concentrations of 1,25(OH) 2 D. CRH and COX-2 mRNA levels were assessed by . We transfected STBs with VDR small interfering RNA (siRNA) and inhibitor of miR-181b2, a miRNA predicted to repress CRH, and treated with 1,25 (OH) 2 D to determine the mechanism of action of vitamin D effects on placental gene expression. RESULTS: VDR, when bound to its ligand 1,25(OH) 2 D, interacted with a total of 1,586 genomic sites including 542 miRNA genes, a number of which were predicted to target labor-associated genes.
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