Simple SummaryAnaemia refers to a low red blood cell count and is common during pregnancy in women. Anaemia has been reported in sheep undergoing surgery during pregnancy for biomedical research projects. The incidence and severity of anaemia during pregnancy in sheep is unknown. Three groups of sheep were established: non-pregnant; pregnant with a single lamb; and pregnant with twin lambs. The stage of pregnancy was known as the sheep were mated on a known date and pregnancy was confirmed 50 days later by ultrasound examination. During pregnancy, blood samples were collected to measure red cells in the blood and protein levels. Samples were also collected to ensure that the sheep did not have any risk factors for anaemia, such as nutritional deficiency and internal parasites. Anaemia did not occur in any sheep during the study. Anaemia did not develop during pregnancy and red cell counts and protein levels were similar between each group. All the sheep lambed as expected, except for one that was expecting twins delivered a single lamb.AbstractThe aim of this study was to document the haematological profile of pregnant ewes throughout gestation. Sheep were divided into three groups (n = 8 per group): non-pregnant, singleton, or twin pregnancy. Blood samples were collected every 14 days from day 55 of gestation for haemoglobin concentration; packed cell volume; total protein; and albumin concentration. On days 55 and 125 of gestation blood was collected for trace element estimation: soluble copper and zinc; glutathione peroxidase (GSHPx); and methylmalonic acid (MMA). Pooled faecal samples were collected on days 55, 97, and 139 of gestation. Pasture cuts were collected on days 97 and 153 of gestation. The haematology and protein concentrations were not different between groups throughout the study. Copper concentration increased in all animals during the study (p < 0.0001). Zinc concentration was lowest in the singleton and twin pregnant sheep on day 55 of gestation (p = 0.04). GSHPx was not different between groups during the study. MMA decreased in all animals during the study (p < 0.0001), but was not different between groups. Faecal samples were consistently negative for strongyle and nematode eggs, and coccidian oocysts. The pasture was good quality. Pregnant sheep in a farm environment with normal trace element status, no parasites, and an adequate diet, did not develop anaemia (PCV < 0.27).
Two Jersey cows were tre<1ted dermally with 32P-labelled fenchlorphos "pour-on" (4% w Iv) at the rate of 7.5 mg active ingredient/kg body weight. Highest residues of total radioactivity appeared in the blood, milk, urine, and faeces on the 2nd day after treatment. The residues were predominantly water-soluble hydrolysis products of fenchlorphos. Maximum concentrations of fenchlorphos and its organosoluble metabolites in milk from the two cows were 0.10 ,ug/ml and 0.13 ,ug/ml on the 2nd day after treatment. Of the total radioactivity applied to each cow, 50-60% was excreted in the urine, 2% in the faeces, and 1 % in the milk over a period of 3 weeks. Radioactivity was still detectable on the backs and flanks of both cows 10 weeks after treatment.
Pastures were topdressed with pellets of 5% fensulfothion (Dasanit) at the rate of 2 lb active ingredient (a.i.)jacre.
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