Our study demonstrates that a maternal multiple-dose AZI regimen is effective in eradicating U. parvum IAI by virtue of intra-amniotic accumulation and suggests that antenatal therapy has the potential to mitigate complications associated with U. parvum infection in pregnancy, such as preterm labor and fetal sequelae.
MicroRNAs (miRNAs) are small, noncoding RNAs that post-transcriptionally regulate gene expression and are recognized for their roles both as modulators of disease progression and as biomarkers of disease activity, including neurological diseases, cancer, and cardiovascular disease (CVD). Commonly, miRNA abundance is assessed using quantitative real-time PCR (qRT-PCR), however, qRT-PCR for miRNA can be labor intensive, time consuming, and may lack specificity for detection of mature versus precursor forms of miRNA. Here, we describe a novel double molecular beacon approach to miRNA assessment that can distinguish and quantify mature versus precursor forms of miRNA in a single assay, an essential feature for use of miRNAs as biomarkers for disease. Using this approach, we found that molecular beacons with DNA or combined locked nucleic acid (LNA)-DNA backbones can detect mature and precursor miRNAs (pre-miRNAs) of low (< 1 nM) abundance in vitro. The double molecular beacon assay was accurate in assessing miRNA abundance in a sample containing a mixed population of mature and precursor miRNAs. In contrast, qRT-PCR and the single molecular beacon assay overestimated miRNA abundance. Additionally, the double molecular beacon assay was less labor intensive than traditional qRT-PCR and had 10-25% increased specificity. Our data suggest that the double molecular beacon-based approach is more precise and specific than previous methods, and has the promise of being the standard for assessing miRNA levels in biological samples.
RÉSUMÉCette analyse documentaire vise à déterminer les principaux éléments du mode d'utilisation des soins médicaux chez les aînés dans le cadre du Consensus national sur la conception et la prestation d'une réforme des soins de santé visant à promouvoir l'autonomie des personnes âgées (Shapiro & Havens, 2000). On y présente une stratégie de recherche de la documentation pertinente depuis 1985 portant principalement sur des articles de revuses révisés par les pairs et on résume les conclusions de la recherche. On souligne les principaux articles, lesquels sont analysés et synthétisés à partir de modalités américaines ou canadiennes de fonctionnement institutionnel, de recours aux médecins, aux soin ambulatoires et sur des déterminants de l'utilisation de ces services.
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