Results show that PDT accelerates the closure of ulcer wounds and to evaluate the wound area, different measurement methods can be used to follow-up the tissue repair process.

RESUMO:O objetivo deste trabalho foi avaliar a atividade antifúngica de óleos essenciais e vegetais no controle in vitro de Colletotrichum gloeosporioides, agente causal da antracnose em pós-colheita de frutíferas. Treze óleos essenciais foram utilizados em concentrações de 0,00%, 0,40%, 0,80%, 1,70%, 3,20%, 6,25%, 12,50%, 25,00%, 50,00% e 100,00%, e uma linhagem padrão de Colletotrichum gloeosporioides. Foram avaliadas a concentração inibitória mínima e a concentração mínima fungicida a fim de caracterizar o potencial de cada um dos óleos essenciais avaliados. Verificou-se que os óleos utilizados apresentaram atividade fungicida em diferentes concentrações, as quais variaram de 0,80% (melaleuca), 3,20%, (eucalipto), 6,25% (limão, capim limão, cravo da índia, canela e nim), 12,5% (hortelã e citronela), 25% (copaíba), 50% (coco e gengibre) e 100% (manjericão). O óleo de nim apresentou maior redução da carga microbiana em função do tempo de exposição, sendo necessários 30 minutos para anulação da contagem microbiana. O efeito antifúngico dos óleos essenciais, para controle de Colletotrichum gloeosporioides, depende da planta e da concentração empregada.Palavras-chave: antracnose, frutíferas, antifúngicos, plantas medicinais.ABSTRACT: Essential and vegetal oils in the in vitro control of Colletotrichum gloeosporioides. This study aimed to evaluate the antifungal effect of essential and vegetal oils in the in vitro control of Colletotrichum gloeosporioides, a causal agent of anthracnose in fruit postharvest. Thirteen essential oils were used at concentrations of 0.00%, 0.40%, 0.80%, 1.70%, 3.20%, 6.25%, 12.50%, 25.00%, 50.00%, and 100.00%, and also a standard strain of Colletotrichum gloeosporioides, The minimum inhibitory concentration and minimum fungicidal concentration were assessed to characterize the potential of each of the essential oils tested. We found that used oils showed fungicidal activity at different concentrations, which varied in 0.80% (Melaleuca alternifólia), 3.20%, (Eucalyptus globulus), 6.25% (Citrus limonium, Cymbopogon citratus, Syzygium aromaticum, Cinnamomum zeylanicum, and Azadirachta indica), 12.5% (Mentha piperita and Cymbopogon winterianus), 25% (Copaifera langsdorfii), 50% (Cocos nucifera and Zingiber officinale), and 100% (Ocimum basilicum). The Azadirachta indica oil showed greater reduction of microbial load because of the exposure time, and took 30 minutes for annulment of microbial count. The antifungal effect of essential oils to control Colletotrichum gloeosporioides depends on the plant and quantity of concentration. INTRODUÇÃOA principal preocupação dos agricultores na produção de frutas está relacionada com a manifestação de doenças na fase de pós-colheita, entre elas, a antracnose, que é causada pelo fungo Colletotrichum gloeosporioides Penz. (Silva et al., 2009).A antracnose é a doença de pós-colheita de maior ocorrência nas regiões tropicais e subtropicais do mundo, podendo atingir frutas como o mamão (Carica papaya L.), a manga (Mangifera indica L.), a banana (Musa spp.)...

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Photodynamic therapy was effective when applied in vivo for subclinical bovine mastitis. There was no need to separate the animal from production.

RESUMO -A adenite equina, popularmente chamada de garrotilho, é uma enfermidade contagiosa, causada pela bactéria Streptococcus equi. A enfermidade é conhecida por ter alta morbidade e baixa letalidade e seus prejuízos econômicos devem-se à perda de performance e alto custo do tratamento. O objetivo deste trabalho foi avaliar a presença e o perfil de resistência a antibióticos de Streptococcus equi da cavidade nasal de equinos hígidos da região de Fernandópolis -SP. Foram utilizados 50 equinos hígidos sem raça definida e com idade entre 12 a 24 meses. De cada animal foram colhidas amostras de exsudato da cavidade nasal por meio de swabs esterilizados e em seguida colocados em tubos contendo ágar base acrescido com 5% de sangue ovino desfibrinado. Os isolados que apresentaram colônias mucosas e β-hemolíticas foram analisados pela coloração de Gram, submetidos ao teste da catalase, e identificados pelo sistema API Rapid ID 32 Strep. Todos os isolados foram avaliados em difusão em placa. Foram isolados sessenta e sete amostras de Streptococcus equi, das subespécies equi e zooepidemicus. Verificou-se prevalência maior de S. equi subesp zooepidemicus (p < 0,001). A maioria dos isolados de S. equi subespécies equi foram mutirresistentes, havendo diferenças significativas entre os diferentes antibióticos (p < 0,005), enquanto que foi verificada maior susceptibilidade nos isolados S. equi subespécies zooepidemicus (p < 0,05). Os resultados obtidos mostraram que ambas as subespécies de Streptococcus equi fazem parte da microbiota normal da cavidade nasal de equinos, e que as mesmas diferem quanto a susceptibilidade aos antibióticos. Palavras-Chave: Adenite equina; equinos; Streptococcus equi; susceptibilidade.ABSTRACT -Strangles is a disease of the respiratory tract of horses caused by Streptococcus equi. The disease has high morbidity and low lethality, and produces economic losses due to low performance and high cost treatment. This research focused on evaluate antibiotic resistance of Streptococcus equi from the nasal cavity of healthy horses from the region Fernandópolis -São Paulo. Fifty (50) mongrel healthy horses, ageing from twelve to twenty four (12-24) months were used. Samples from the nasal cavity of each animal were obtained using sterile swabs and after were cultured on blood agar base plus 5% defibrinated sheep blood. Plates that showed mucous colonies and β -hemolytic were tested using Gram stain method, the catalase test, and identified by the system API Rapid ID thirty-two 32 Strep. All isolates were analyzed by plate difusion. Sixty seven of the obtained isolates were Streptococcus equi subspecies equi and zooepidemicus. There was higher prevalence of S. subsp equi zooepidemicus (p < 0.001). Most isolates of S. equi subsp equi were antibiotical multiresistant with significant differences between different antibiotics (p < 0.005), whereas higher susceptibility was observed in isolated S. subsp equi zooepidemicus (p < 0.05). The results showed that both subspecies of Streptococcus equi are part of the no...

Introduction: Ozone is a potent antibacterial agent. Because ozone oxidizes organic material, it directly attacks microorganisms resulting in safe, fast and economical sterilization at low temperatures. This study evaluated the effi cacy of ozonated water in the reprocessing of dialyzers obtained from a hemodialysis service in São José do Rio Preto. Methods: A total of 23 blood dialyzers were collected at the conclusion of the morning hemodialysis shift. The equipment was collected after the maximum number of reuses, with the last disinfection performed with purifi ed water (obtained by reverse osmosis and subsequent reverse ultrafi ltration). The number and species of microorganisms in the dialyzers were evaluated before and after treatment with ozonated water. The ozonation of sterile deionized water was achieved by direct contact between the water and the ozone generating equipment. Results: The mean number of microorganisms before sterilization was 1,47.10 9 colony forming units per ml (CFU/ml). After treatment with ozonated water, the number of microorganisms was 4,80.10 1 CFU/ml. Conclusion: Ozonated water is an effective decontaminant for most blood dialyzers.

Sporotrichosis is a common disease in tropical regions, caused by the fungus Sporothrix schenckii, affecting mainly rural workers and in direct contact with animals. Although treatment by indiscriminate use of oral antifungal drugs gives rise resistant isolates, leading to therapeutic failures and no remission of the disease. To evaluate the effectiveness of red low-power laser photobiomodulation in inactivation of S. schenckii infection induced in rodents. Methods: Subcutaneously inoculation (2x10 3 S. schenckii/ml, 0.2 ml suspension) in the left footpad, in 27 mice divided into: control (n = 6, inoculated, without irradiation): early stage (not inoculated) = 1 th biopsy; intermediate (9 weeks of evolution) = 2 nd biopsy; final (21 weeks of evolution) = 3 th biopsy. Treated (n = 21, inoculated and irradiated): early (13 weeks of evolution, 4 weeks after first irradiation) = 4 th biopsy, intermediate (17 weeks of evolution, 8 weeks after first irradiation) = 5 th biopsy, final (21 weeks of evolution, 12 weeks after first irradiation) = 6 th biopsy. Serial irradiation with biopsies occurred every 30 days during each month, for three months. At the end of testing, the mice were euthanized, and histological analyzes of biopsies were performed. Results: Each laser treatment session showed an inactivation of S. schenckii in treated animals compared to controls, with a regression of pseudoepitheliomatous hyperplasia, chronic inflammation, neutrophils, granulomas, giant mononuclear cells and steroid corpuscles. Conclusion: The laser photobiomodulation was effective on S. schenckii inactivation, appearing to be an interesting therapeutic option in infections caused by this organism.

BACKGROUNDDermatophytes are filamentous keratinophilic fungi. Trichophyton rubrum is a prevalent infectious agent in tineas and other skin diseases. Drug therapy is considered to be limited in the treatment of such infections, mainly due to low accessibility of the drug to the tissue attacked and development of antifungal resistance in these microorganisms. In this context, Photodynamic Therapy is presented as an alternative.OBJECTIVEEvaluate, in vitro, the photodynamic activity of four derivatives of Protoporphyrin IX by irradiation with LED 400 nm in T. rubrum.METHODAssays were subjected to irradiation by twelve cycles of ten minutes at five minute intervals.RESULTPhotodynamic action appeared as effective with total elimination of UFCs from the second irradiation cycle.CONCLUSIONStudies show that the photodynamic activity on Trichophyton rubrum relates to a suitable embodiment of the photosensitizer, which can be maximized by functionalization of peripheral groups of the porphyrinic ring.