BackgroundCassava, Manihot esculenta Crantz, is one of the most important crops world-wide representing the staple security for more than one billion of people. The development of dense genetic and physical maps, as the basis for implementing genetic and molecular approaches to accelerate the rate of genetic gains in breeding program represents a significant challenge. A reference genome sequence for cassava has been made recently available and community efforts are underway for improving its quality. Cassava is threatened by several pathogens, but the mechanisms of defense are far from being understood. Besides, there has been a lack of information about the number of genes related to immunity as well as their distribution and genomic organization in the cassava genome.ResultsA high dense genetic map of cassava containing 2,141 SNPs has been constructed. Eighteen linkage groups were resolved with an overall size of 2,571 cM and an average distance of 1.26 cM between markers. More than half of mapped SNPs (57.4%) are located in coding sequences. Physical mapping of scaffolds of cassava whole genome sequence draft using the mapped markers as anchors resulted in the orientation of 687 scaffolds covering 45.6% of the genome. One hundred eighty nine new scaffolds are anchored to the genetic cassava map leading to an extension of the present cassava physical map with 30.7 Mb. Comparative analysis using anchor markers showed strong co-linearity to previously reported cassava genetic and physical maps. In silico based searching for conserved domains allowed the annotation of a repertory of 1,061 cassava genes coding for immunity-related proteins (IRPs). Based on physical map of the corresponding sequencing scaffolds, unambiguous genetic localization was possible for 569 IRPs.ConclusionsThis is the first study reported so far of an integrated high density genetic map using SNPs with integrated genetic and physical localization of newly annotated immunity related genes in cassava. These data build a solid basis for future studies to map and associate markers with single loci or quantitative trait loci for agronomical important traits. The enrichment of the physical map with novel scaffolds is in line with the efforts of the cassava genome sequencing consortium.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-1397-4) contains supplementary material, which is available to authorized users.

Pathogenesis-related (PR) proteins are inducible antimicrobial defence proteins whose specific function in immunity or in other plant processes have not been deeply studied. Genes coding for PRs have been reported in different plant species with numerous homologous proteins belonging to the same functional protein family. Previously some PR candidate genes were identified in response to cassava bacterial blight (CBB) caused by Xanthomonas axonopodis pv. manihotis (Xam): the genes for hevamine (HEV), chitinase (CHI) and sulphite reductase (SiR) were induced in a resistant cassava cultivar during the response to Xam. To understand their functional roles in defence responses, a protein-protein interaction map was generated based on yeast two-hybrid (Y2H) assays of these candidate PR proteins. The results showed that the cassava PRs interact with each other and, although hevamine and chitinase belong to the same class of chitinases, they share only four interactors. Co-regulated expression of PRs and their interactors was observed and similar ontology terms were identified for interactors. Furthermore, an overlap between immune and other metabolic pathways was noticed based on other bioinformatics studies. These results therefore shed more light on the possible function of PR proteins.

One of the most challenging questions in plant breeding and molecular plant pathology research is what are the genetic and molecular bases of quantitative disease resistance (QDR)?. The scarce knowledge of how this type of resistance works has hindered plant breeders to fully take advantage of it. To overcome these obstacles new methodologies for the study of quantitative traits have been developed. Approaches such as genetic mapping, identification of quantitative trait loci (QTL) and association mapping, including candidate gene approach and genome wide association studies, have been historically undertaken to dissect quantitative traits and therefore to study QDR. Additionally, great advances in quantitative phenotypic data collection have been provided to improve these analyses. Recently, genes associated to QDR have been cloned, leading to new hypothesis concerning the molecular bases of this type of resistance. In this review we present the more recent advances about QDR and corresponding application, which have allowed postulating new ideas that can help to construct new QDR models. Some of the hypotheses presented here as possible explanations for QDR are related to the expression level and alternative splicing of some defense-related genes expression, the action of "weak alleles" of R genes, the presence of allelic variants in genes involved in the defense response and a central role of kinases or pseudokinases. With the information recapitulated in this review it is possible to conclude that the conceptual distinction between qualitative and quantitative resistance may be questioned since both share important components. Keywords: breeding, complex traits, genome, gene expression, plant immunity, quantitative disease resistance (QDR), quantitative trait loci (QTL). RESUMENUna de las preguntas más desafiantes del fitomejoramiento y de la fitopatología molecular es ¿cuáles son las bases genéticas y moleculares de la resistencia cuantitativa a enfermedades?. El escaso conocimiento de cómo este tipo de resistencia funciona ha obstaculizado que los fitomejoradores la aprovecharlo plenamente. Para superar estos obstáculos se han desarrollado nuevas metodologías para el estudio de rasgos cuantitativos. Los enfoques como el mapeo genético, la identificación de loci de rasgos cuantitativos (QTL) y el mapeo por asociaciones, incluyendo el enfoque de genes candidatos y los estudios de asociación amplia del genoma, se han llevado a cabo históricamente para describir rasgos cuantitativos y por lo tanto para estudiar QDR. Además, se han proporcionado grandes avances en la obtención de datos fenotípicos cuantitativos para mejorar estos análisis. Recientemente, algunos genes asociados a QDR han sido clonados, lo que conduce a nuevas hipótesis sobre las bases moleculares de este tipo de resistencia. En esta revisión presentamos los avances más recientes sobre QDR y la correspondiente aplicación, que han permitido postular nuevas ideas que pueden ayudar a construir nuevos modelos. Algunas de las hipótesis presen...

We conducted a SSRs density analysis in different cassava genomic regions. The information obtained was useful to establish comparisons between cassava's SSRs genomic distribution and those of poplar, flax, and Jatropha. In general, cassava has a low SSR density (~50 SSRs/Mbp) and has a high proportion of pentanucleotides, (24,2 SSRs/Mbp). It was found that coding sequences have 15,5 SSRs/Mbp, introns have 82,3 SSRs/Mbp, 5′ UTRs have 196,1 SSRs/Mbp, and 3′ UTRs have 50,5 SSRs/Mbp. Through motif analysis of cassava's genome SSRs, the most abundant motif was AT/AT while in intron sequences and UTRs regions it was AG/CT. In addition, in coding sequences the motif AAG/CTT was also found to occur most frequently; in fact, it is the third most used codon in cassava. Sequences containing SSRs were classified according to their functional annotation of Gene Ontology categories. The identified SSRs here may be a valuable addition for genetic mapping and future studies in phylogenetic analyses and genomic evolution.

Background Fusarium culmorum is an important pathogen causing head blight of cereals in Europe. This disease is of worldwide importance leading to reduced yield, grain quality, and contamination by mycotoxins. These mycotoxins are harmful for livestock and humans; therefore, many countries have strict regulatory limits for raw materials and processed food. Extensive genetic diversity is described among field populations of F. culmorum isolates for aggressiveness and production of the trichothecene mycotoxin deoxynivalenol (DON). However, the causes for this quantitative variation are not clear, yet. We analyzed 92 isolates sampled from different field populations in Germany, Russia, and Syria together with an international collection for aggressiveness and DON production in replicated field experiments at two locations in two years with two hosts, wheat and rye. The 30x coverage whole-genome resequencing of all isolates resulted in the identification of 130,389 high quality single nucleotide polymorphisms (SNPs) that were used for the first genome-wide association study in this phytopathogenic fungus. Results In wheat, 20 and 27 SNPs were detected for aggressiveness and DON content, respectively, of which 10 overlapped. Additionally, two different SNPs were significantly associated with aggressiveness in rye that were among those SNPs being associated with DON production in wheat. Most of the SNPs explained only a small proportion of genotypic variance (pG), however, four SNPs were associated with major quantitative trait loci (QTLs) with pG ranging from 12 to 48%. The QTL with the highest pG was involved in DON production and associated with a SNP most probably located within the Tri4 gene. Conclusions The diversity of 92 isolates of F. culmorum were captured using a heuristic approach. Key phenotypic traits, SNPs, and candidate genes underlying aggressiveness and DON production were identified. Clearly, many QTLs are responsible for aggressiveness and DON content in wheat, both traits following a quantitative inheritance. Several SNPs involved in DON metabolism, among them the Tri4 gene of the trichothecene pathway, were inferred as important source of variation in fungal aggressiveness. Using this information underlying the phenotypic variation will be of paramount importance in evaluating strategies for successful resistance breeding.

Introducción: la diabetes tipo 2 (DM2) es una patología que se desarrolla progresivamente ante la presencia de factores de riesgo (FR) predisponentes y que deberían identificarse previo a su diagnóstico.Objetivos: detectar e identificar los FR para DM2 en población adulta de la provincia de Neuquén a fin de realizar prevención primaria para DM2.Materiales y métodos: estudio descriptivo de 1.232 encuestas anónimas realizadas en la provincia de Neuquén entre 2017 y 2018, dirigidas a adultos ≥20 años sin diagnóstico conocido de DM2, en 10 ciudades del territorio neuquino. Las mismas fueron contestadas por autorreporte (AR) o por encuestador (ENC) adiestrado a tal efecto y que realizaron medidas antropométricas. Se dispusieron encuestas con urnas en reparticiones municipales, provinciales, privadas, escuelas, farmacias y salas de espera de centros asistenciales.La misma se basó en la encuesta FINDRISC original que estima el riesgo de desarrollar DM2 en los siguientes 10 años. También se incluyeron 4 (cuatro) preguntas adicionales, sin puntaje. Todas las encuestas fueron remitidas a un mismo lugar para su análisis estadístico.Variables originales FINDRISC: Sexo: femenino (F) y masculino (M). Grupo etario (GE): <45, 45-54, 55-64, >65 años, puntaje (P) 0-2-3-4. Índice de masa corporal (IMC): <25, 25-30, >30 P 0-1-4. Circunferencia de cintura (CC) P: 0-3-4 sexo F: <80, 80-88, >88 cm, sexo M: <94, 94-102, >102 cm. Actividad física (AF) 30 minutos/día SÍ/NO, P 0-2. Consumo de vegetales/frutas (VyF) SÍ/NO con P 0-2. Antecedente de Azúcar elevada en Sangre (AGluc) NO/SÍ P 0-5. Antecedente Familiar con DM 1 ó 2 (AFDM): NO, familiar en 2° ó en 1°, con P 0-3-5. HTA: NO/SI.Categorías de riesgo del FINDRISC (R): Bajo (RB) <7 P, ligeramente elevado (RLE) 7-11 P, moderado (RM) 12-14 P, alto (RA) 15-20 P, muy alto (RMA) >20 P.Variables agregadas, sin puntaje: tabaquismo (Tab), colesterol elevado (Col), hijo nacido de ≥4 Kg de peso (RNAP) y familiar con ACV/IAM (FECV), respuestas: NO/SÍ.La encuesta incluye un troquel que la persona debía conservar y donde se lo invita a hacer consulta médica en caso de tener P ≥15. La encuesta en sí debía ser depositada en la urna.