The emergence of SARS-CoV-2 variants of concern suggests viral adaptation to enhance human-to-human transmission1,2. Although much effort has focused on the characterization of changes in the spike protein in variants of concern, mutations outside of spike are likely to contribute to adaptation. Here, using unbiased abundance proteomics, phosphoproteomics, RNA sequencing and viral replication assays, we show that isolates of the Alpha (B.1.1.7) variant3 suppress innate immune responses in airway epithelial cells more effectively than first-wave isolates. We found that the Alpha variant has markedly increased subgenomic RNA and protein levels of the nucleocapsid protein (N), Orf9b and Orf6—all known innate immune antagonists. Expression of Orf9b alone suppressed the innate immune response through interaction with TOM70, a mitochondrial protein that is required for activation of the RNA-sensing adaptor MAVS. Moreover, the activity of Orf9b and its association with TOM70 was regulated by phosphorylation. We propose that more effective innate immune suppression, through enhanced expression of specific viral antagonist proteins, increases the likelihood of successful transmission of the Alpha variant, and may increase in vivo replication and duration of infection4. The importance of mutations outside the spike coding region in the adaptation of SARS-CoV-2 to humans is underscored by the observation that similar mutations exist in the N and Orf9b regulatory regions of the Delta and Omicron variants.

We present a random forest-based framework for real time head pose estimation from depth images and extend it to localize a set of facial features in 3D. Our algorithm takes a voting approach, where each patch extracted from the depth image can directly cast a vote for the head pose or each of the facial features. Our system proves capable of handling large rotations, partial occlusions, and the noisy depth data acquired using commercial sensors. Moreover, the algorithm works on each frame independently and achieves real time performance without resorting to parallel computations on a GPU. We present extensive experiments on publicly available, challenging datasets and present a new annotated head pose database recorded using a Microsoft Kinect.

We present a novel approach for detecting social interactions in a crowded scene by employing solely visual cues. The detection of social interactions in unconstrained scenarios is a valuable and important task, especially for surveillance purposes. Our proposal is inspired by the social signaling literature, and in particular it considers the sociological notion of F-formation. An F-formation is a set of possible configurations in space that people may assume while participating in a social interaction. Our system takes as input the positions of the people in a scene and their (head) orientations; then, employing a voting strategy based on the Hough transform, it recognizes F-formations and the individuals associated with them. Experiments on simulations and real data promote our idea.

No abstract

In this chapter, we propose a comparison between two techniques for one-shot person re-identification from soft biometric cues. One is based upon a descriptor composed of features provided by a skeleton estimation algorithm; the other compares body shapes in terms of whole point clouds. This second approach relies on a novel technique we propose to warp the subject's point cloud to a standard pose, which allows to disregard the problem of the different poses a person can assume. This technique is also used for composing 3D models which are then used at testing time for matching unseen point clouds. We test the proposed approaches on an existing RGB-D re-identification dataset and on the newly built BIWI RGBD-ID dataset. This dataset provides sequences of RGB, depth, and skeleton data for 50 people in two different scenarios and it has been made publicly available to foster advancement in this new research branch.

No abstract

No abstract

No abstract