Antibiotic resistance results in higher medical costs, prolonged hospital stays and increased mortality and is rising to dangerously high levels in all parts of the world. Therefore, this study aims to search for new antimicrobial agents through bioprospecting of extracts of endophytic fungi from Bauhinia guianensis, a typical Amazonian plant used in combating infections. Seventeen (17) fungi were isolated and as result the methanolic extract of the fungus Exserohilum rostratum showed good activity against the bacteria tested. The polyketide monocerin was isolated by the chromatographic technique, identified by NMR and MS, showing broad antimicrobial spectrum.
Leishmaniasis is a neglected infectious disease caused by different species of the Leishmania parasite and is one of the major public health problems in developing countries. Despite the progress in fundamental knowledge about the Leishmania parasite, current therapy against leishmaniasis is still unsatisfactory due to limited efficacy, prolonged treatment, high cost and undesirable adverse effects. Thus, the research for new prototypes compounds of antileishmaniasis drugs remains a matter of current importance. The objective of this study was to evaluate the leishmanicidal activity of cytochalasin B, a natural compound isolated by our group in a previous study, against L. amazonensis, using experimental tests and computational simulation methodologies. The results of the biological evaluation showed that cytochalasin B has antileishmanial activity against the promastigote form of Leishmania amazonensis. These results are corroborated by the docking and molecular dynamics that showed that the activity occurs due to the inactivation of the trypanothione reductase enzyme (TryR).
Constituintes Químicos e Atividade Antimicrobiana do Fungo de Solo Aspergillus sp. FRIZ12 Resumo: O presente trabalho relata o isolamento de nove compostos da biomassa do fungo de solo Aspergillus sp. FRIZ12. Os compostos 2-benzil-4H-piran-4-ona (1), flavasperona (2), 5-(hidroximetil) furfural (3), uridina (4), ergosterol (5), peróxido de ergosterol (6), cerivisterol (7), estigmasterol (8) e sitosterol (9) foram isolados por métodos cromatográficos, e as estruturas foram identificadas por RMN, EM e comparação com dados da literatura. O composto 1 está sendo relatado pela primeira vez no gênero Aspergillus e pela segunda vez como um produto natural. Os compostos 1 e 2 mostraram moderada atividade antimicrobiana
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