Chemical Constituents and Antimicrobial Activity of Soil Fungus Aspergillus sp. FRIZ12

Pinheiro, Eduardo Antonio Abreu; Pina, Jeferson Rodrigo Souza; Feitosa, André O.; Carvalho, Josiwander Miranda; Marinho, Patrícia Santana Barbosa; Marinho, Andrey Moacir do Rosário

doi:10.21577/1984-6835.20180097

Cited by 2 publications

(1 citation statement)

References 15 publications

(23 reference statements)

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“…Our result revealed that the extract and the compounds exert a significant anti-inflammatory effect and the most potent one is compound 2 as previously reported that butenolides can exhibit anti-inflammatory activity (Qia et al 2018 ). Moreover, it was reported that the extract obtained from A. terreus showed an antimicrobial activity against different strains of microorganisms (Pinheiro et al 2018 ). The biological activity of the extract may be due to the synergistic activity of the different compounds present in the extract.…”

Section: Discussionmentioning

confidence: 99%

Bioactive metabolites identified from Aspergillus terreus derived from soil

Fayek,

Ebrahim,

Abdel-Aziz

et al. 2023

AMB Expr

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Aspergillus terreus has been reported to produce many bioactive metabolites that possess potential activities including anti-inflammatory, cytotoxic, and antimicrobial activities. In the present study, we report the isolation and identification of A. terreus from a collected soil sample. The metabolites existing in the microbial ethyl acetate extract were tentatively identified by HPLC/MS and chemically categorized into alkaloids, terpenoids, polyketides, γ-butyrolactones, quinones, and peptides. In addition, a new triglyceride (1) and a diketopiperazine derivative namely asterrine (4), together with two known butyrolactone (2–3) were purified from the extract. The chemical skeleton of the purified compounds was established by comprehensive analysis of their ESI/MS, 1 and 2D-NMR data. The extract and compounds 3,4 exhibited a strong inhibitory activity for the binding of ACE2 to SARS-CoV-2 spike-protein receptor with IC50 7.4, 9.5, and 8.5 µg/mL, respectively. In addition, the extract, 1 and 2 displayed a potent anti-inflammatory effect with IC50 51.31 and 37.25 pg/mL (Il-6) and 87.97, 68.22 pg/mL (TNF-α), respectively, in comparison to LPS control. In addition, the extract and compound 4 displayed an antimicrobial effect towards S. aureus by MIC 62.5 and 125 μg/mL, while the extract exhibited a potent effect against C. albicans (MIC of 125 μg/mL). Collectively, our data introduce novel bioactivities for the secondary metabolites produced by the terrestrial fungus Aspergillus terreus.

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Section: Discussionmentioning

confidence: 99%

Bioactive metabolites identified from Aspergillus terreus derived from soil

Fayek,

Ebrahim,

Abdel-Aziz

et al. 2023

AMB Expr

View full text Add to dashboard Cite

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Promising Cytotoxic butenolides from the Soybean endophytic fungus Aspergillus terreus: a combined molecular docking and in-vitro studies

El‐Hawary

Moawad

Bahr

et al. 2023

Journal of Applied Microbiology

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Aim This study aimed to use one strain many compounds approach (OSMAC) to investigate the cytotoxic potential of Aspergillus terreus associated with soybean versus several cancer cell lines, by means of in-silico and in vitro approaches. Methods and results Fermentation of the isolated strain was done on five media. The derived extracts were investigated for their inhibitory activities against three human cancer cell lines; mammary gland breast cancer (MCF-7), colorectal adenocarcinoma (Caco-2) and hepatocellular carcinoma (HepG2) using MTT Assay. The fungal mycelia fermented in Modified Potato Dextrose Broth (MPDB) was the most cytotoxic extract against HepG2, MCF-7 and Caco-2 cell lines with IC50 4.2 ± 0.13, 5.9 ± 0.013 and 7.3 ± 0.004 µg.mL−1, respectively. MPDB extract was scaled up resulting in the isolation of six metabolites; three fatty acids (1, 2 and 4), one sterol (3) and two butenolides (5 and 6) by column chromatography. The isolated compounds (1–6) were screened through a molecular docking approach for their binding aptitude to various active sites. butyrolactone-I (5) revealed a significant interaction within the CDK2 active site, while aspulvinone E (6) showed promising binding affinity to FLT3 and EGFR active sites that was confirmed by in vitro CDK2, FLT3 and EGFR inhibitory activity. Finally, the in vitro cytotoxic activities of butyrolactone-I (5) and aspulvinone E (6) revealed the antiproliferative activity of butyrolactone-I (5), against HepG2 cell line (IC50 = 17.85 ± 0.32 µM). Conclusion Molecular docking analysis and in vitro assays suggested the CDK2/A2 inhibitory potential of butyrolactone-I (5) in addition to the promising interaction abilities of aspulvinone E (6) with EGFR and FLT3 active sites as a possible mechanism of their biological activities.

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Chemical Constituents and Antimicrobial Activity of Soil Fungus Aspergillus sp. FRIZ12

Cited by 2 publications

References 15 publications

Bioactive metabolites identified from Aspergillus terreus derived from soil

Bioactive metabolites identified from Aspergillus terreus derived from soil

Promising Cytotoxic butenolides from the Soybean endophytic fungus Aspergillus terreus: a combined molecular docking and in-vitro studies

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