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BackgroundDrusen are common features in the ageing macula associated with exudative Age-Related Macular Degeneration (ARMD). They are visible in retinal images and their quantitative analysis is important in the follow up of the ARMD. However, their evaluation is fastidious and difficult to reproduce when performed manually.MethodsThis article proposes a methodology for Automatic Drusen Deposits Detection and quantification in Retinal Images (AD3RI) by using digital image processing techniques. It includes an image pre-processing method to correct the uneven illumination and to normalize the intensity contrast with smoothing splines. The drusen detection uses a gradient based segmentation algorithm that isolates drusen and provides basic drusen characterization to the modelling stage. The detected drusen are then fitted by Modified Gaussian functions, producing a model of the image that is used to evaluate the affected area.Twenty two images were graded by eight experts, with the aid of a custom made software and compared with AD3RI. This comparison was based both on the total area and on the pixel-to-pixel analysis. The coefficient of variation, the intraclass correlation coefficient, the sensitivity, the specificity and the kappa coefficient were calculated.ResultsThe ground truth used in this study was the experts' average grading. In order to evaluate the proposed methodology three indicators were defined: AD3RI compared to the ground truth (A2G); each expert compared to the other experts (E2E) and a standard Global Threshold method compared to the ground truth (T2G).The results obtained for the three indicators, A2G, E2E and T2G, were: coefficient of variation 28.8 %, 22.5 % and 41.1 %, intraclass correlation coefficient 0.92, 0.88 and 0.67, sensitivity 0.68, 0.67 and 0.74, specificity 0.96, 0.97 and 0.94, and kappa coefficient 0.58, 0.60 and 0.49, respectively.ConclusionsThe gradings produced by AD3RI obtained an agreement with the ground truth similar to the experts (with a higher reproducibility) and significantly better than the Threshold Method. Despite the higher sensitivity of the Threshold method, explained by its over segmentation bias, it has lower specificity and lower kappa coefficient. Therefore, it can be concluded that AD3RI accurately quantifies drusen, using a reproducible method with benefits for ARMD evaluation and follow-up.

Background/Aims: To monitor possible changes in the cumulated drusen or geographic atrophy area size (CDGAS) of nonexudative age-related macular degeneration (AMD) in patients before and after cataract surgery, using a new tool for computer-aided image quantification. Methods: Randomized, prospective, clinical trial. 54 patients with cataract and nonexudative AMD were randomly assigned into an early surgery group (ES = 28) and a control group (CO = 26) with a 6-month delay of surgery. CDGAS was determined with the MD3RI tool for contour drawing in a central region of digitized fundus photographs, measuring 3,000 µm in diameter. To evaluate CDGAS progression, differences in pixels and square millimeters were calculated by equivalent tests. Results: Forty-nine patients completed the visits over the 12-month period (ES = 27 and CO = 22). Mean pixel values increased from 201.5 (11.33 × 10^–3 mm²) to 202.7 (11.39 × 10^–3 mm²) in the ES group and from 191.6 (10.77 × 10^–3 mm²) to 194.6 (10.94 × 10^–3 mm²) in the CO group. Finally, equivalence of CDGAS differences between ES and CO could be demonstrated. No exudative AMD was recorded during the study period. Conclusion: In our cohorts, no significant changes were found in CDGAS 12 months after cataract surgery. The MD3RI software could serve as an efficient, precise and objective tool for AMD quantification and monitoring in future trials.

Motivation: Cell division in Escherichia coli is morphologically symmetric. However, as unwanted protein aggregates are segregated to the cell poles and, after divisions, accumulate at older poles, generate asymmetries in sister cells' vitality. Novel single-molecule detection techniques allow observing aging-related processes in vivo, over multiple generations, informing on the underlying mechanisms. Results: CellAging is a tool to automatically extract information on polar segregation and partitioning in division of aggregates in E.coli, and on cellular vitality. From time-lapse, parallel brightfield and fluorescence microscopy images, it performs cell segmentation, alignment of brightfield and fluorescence images, lineage construction and pole age determination, and it computes aging-related features. We exemplify its use by analyzing spatial distributions of fluorescent protein aggregates from images of cells across generations. Availability: CellAging, instructions and an example are available at

This article discusses how computational intelligence techniques are applied to fuse spectral images into a higher level image of land cover distribution for remote sensing, specifically for satellite image classification. We compare a fuzzy-inference method with two other computational intelligence methods, decision trees and neural networks, using a case study of land cover classification from satellite images. Further, an unsupervised approach based on k-means clustering has been also taken into consideration for comparison. The fuzzy-inference method includes training the classifier with a fuzzy-fusion technique and then performing land cover classification using reinforcement aggregation operators. To assess the robustness of the four methods, a comparative study including three years of land cover maps for the district of Mandimba, Niassa province, Mozambique, was undertaken. Our results show that the fuzzy-fusion method performs similarly to decision trees, achieving reliable classifications; neural networks suffer from overfitting; while k-means clustering constitutes a promising technique to identify land cover types from unknown areas.

The information generated by a computer vision system capable of labelling a land surface as water, vegetation, soil or other type, can be used for mapping and decision making. For example, an unmanned aerial vehicle (UAV) can use it to find a suitable landing position or to cooperate with other robots to navigate across an unknown region. Previous works on terrain classification from RGB images taken onboard of UAVs shown that only static pixel-based features were tested with a considerable classification error. This paper proposes a robust and efficient computer vision algorithm capable of classifying the terrain from RGB images with improved accuracy. The algorithm complement the static image features with dynamic texture patterns produced by UAVs rotors downwash effect (visible at lower altitudes) and machine learning methods to classify the underlying terrain. The system is validated using videos acquired onboard of a UAV.

The difficult job of fighting fires and the nearly impossible task to stop a wildfire without great casualties requires an imperative implementation of proactive strategies. These strategies must decrease the number of fires, the burnt area and create better conditions for the firefighting. In this line of action, the Portuguese Institute of Nature and Forest Conservation defined a fire break network (FBN), which helps controlling wildfires. However, these fire breaks are efficient only if they are correctly maintained, which should be ensured by the local authorities and requires verification from the national authorities. This is a fastidious task since they have a large network of thousands of hectares to monitor over a full year. With the increasing quality and frequency of the Earth Observation Satellite imagery with Sentinel-2 and the definition of the FBN, a semi-automatic remote sensing methodology is proposed in this article for the detection of maintenance operations in a fire break. The proposed methodology is based on a time-series analysis, an object-based classification and a change detection process. The change detection is ensured by an artificial neural network, with reflectance bands and spectral indices as features. Additionally, an analysis of several bands and spectral indices is presented to show the behaviour of the data during a full year and in the presence of a maintenance operation. The proposed methodology achieved a relative error lower than 4% and a recall higher than 75% on the detection of maintenance operations.

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