The Amazon region comprises a plethora of fruit-bearing species of which a large number are still agriculturally unimportant. Because fruit consumption has been attributed to an enhanced physical well-being, interest in the knowledge of the chemical composition of underexplored exotic fruits has increased during recent years. This paper provides a comprehensive identification of the polyphenolic constituents of four underutilized fruits from the Amazon region by HPLC/DAD-ESI-MS(n). Araçá ( Psidium guineense ), jambolão ( Syzygium cumini ), muruci ( Byrsonima crassifolia ), and cutite ( Pouteria macrophylla ) turned out to be primarily good sources of hydrolyzable tannins and/or flavonols. Additionally, different flavanonols and proanthocyanidins were identified in some fruits. The antioxidant capacity was determined by using the total oxidant scavenging capacity (TOSC) assay. Cutite showed the highest antioxidant capacity followed by jambolão, araçá, and muruci.

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Changes in chemical composition of coconut (Cocos nucifera) water, including total and soluble solids, titratable acidity (as citric acid), turbidity, ash, lipids and sugars, were investigated in four varieties of coconuts at four stages of maturity of the fruit. The most significant change was observed in the volume of nut water, which increased during development from 233 to 504 ml, with the greatest quantity found at 9 months. Fat, protein, soluble solids, acidity and turbidity also increased steadily with maturity, while pH and ash showed variation throughout maturation. The interaction of variety and stage of maturity of the fruit appeared to have a significant effect on the chemical composition of the coconut water. INTRODUCTIONThe coconut (Cocos nucifera L) is a perennial plant that generally flowers monthly and takes approximately one year for the fruit (nut) to reach full maturity. The plant bears nuts continuously for up to 60-70 years, 12-13 times a year. Apart from fat, coconuts also provide coconut water, which is bottled and sold on the market in Jamaica. Farmers generally harvest the nut at around 9 months when the jelly is less than 0.5 cm thick, soft and translucent; beyond 9-months, the jelly hardens and the volume of water begins to decrease. 1 There has been very little research on coconut water quality from different varieties in the Caribbean. In the present study, the changes in chemical composition of nut water with maturity of Tall and Dwarf varieties in Jamaica were evaluated.

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The potential of fruits occurring in the Amazon with regard to their antioxidant capacity is being addressed increasingly during the last years. In this paper, the antioxidant capacity of fruits from Pouteria macrophylla (cutite) and the compounds which are responsible for that functional property were studied. Diphenyl-picrylhydrazyl (DPPH) and total oxidant scavenging capacity (TOSC) assays were used for the evaluation of antioxidant capacity of fruit aqueous extracts. TOSC assay showed inhibitory eVects against peroxyl (TOSC 50% = 560 mg/L) and peroxynitrite (TOSC 50% = 830 mg/L), two important reactive oxygen species, while DPPH showed that cutite (EC 50 = 300 § 3.9 g/g DPPH) has signiWcant antioxidant capacity, greater than many other known Amazon fruits. It was also seen that the cutite fruits are a good alimentary source of polyphenols (867 § 8 mg GAE/100 g), analyzed by Folin-Ciocalteu assay. Using HPLC-DAD-ESI-MS n and HPLC-DAD, gallic acid (4.72 mg/g fresh fruit pulp) and digalloyl glucose were found as the main phenolic compounds in the cutite fruits. TOSC assays with HPLC fractions of fruit aqueous extracts show that both compounds are predominantly responsible for the antioxidant capacity observed in the cutite.

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Exotic fruits such as açai, camu-camu, and blackberries rich in natural antioxidants (ascorbic acid, anthocyanins) are marketed as "functional" foods supporting a pro-/antioxidant balance. Confirming data from human studies are lacking. Within a randomized controlled crossover trial, 12 healthy nonsmokers ingested 400 mL of a blended juice of these fruits or a sugar solution (control). Blood was drawn before and afterward to determine antioxidants in plasma, markers of antioxidant capacity [trolox equivalent antioxidant capacity, Folin-Ciocalteu reducing capacity, total oxidant scavenging capacity (TOSC)] and oxidative stress [isoprostane, DNA strand breaks in leukocytes in vivo], and their resistance versus H₂O₂-induced strand breaks. Compared with sugar solution, juice consumption increased plasma ascorbic acid and maintained TOSC and partly Folin-Ciocalteu reducing capacity (both P values < 0.05). Strand breaks in vivo increased after ingestion of both beverages (P < 0.001), probably due to postprandial and/or circadian effects. This anthocyanin-rich fruit juice may stabilize the pro-/antioxidant balance in healthy nonsmokers without affecting markers of oxidative stress.