Twelve clinically healthy, adult male dogs randomly equally divided into two groups (I and II). Animals of both the groups received xylazine-ketamine anesthesia. Laparoscopic bilateral vasectomy was performed in group I, whereas in animals of group II in additition to vasectomy, spermatic artery-vein plexus were clipped with titanium clips at a distance of 1 to 2 cm. Insufflation of abdominal cavity was achieved by CO 2 (2 liter/minute) at 10 mm Hg pressure gradient. Clinical observations revealed no significant changes. Differential leukocyte count (DLC) revealed significant neutrophilia and comparative lymphopenia on 3rd postoperative in both groups. Significant increase (p < 0.05) in plasma alkaline and acid phosphatase level was observed on day 3 postoperatively. Indices of oxidative stress viz lipid peroxidation (LPO), catalase (CAT), superoxide dismutase (SOD), reduced glutathione activity and acute phase protein, ceruloplasmin level in plasma did not revealed any major significant changes but indicated that oxidative stress was more in group II animals. Plasma cortisol level increased significantly (p < 0.01) after operation and testosterone level showed gradual decrease (p > 0.05) up to 7th postoperative day in animals of group II. On the basis of the parameters studied, it can be concluded that capnoperitoneum at 10 mm Hg pressure gradient and CO 2 at the flow rate of 2 liter/minute provides optimum visualization of intra-abdominal organs and found suitable for laparoscopic sterilization in male dogs. The laparoscopic vasectomy alone in male dogs was found comparatively quick, less time consuming and can be successfully applied for mass sterilization program. Oxidative stress in laparoscopic vasectomy (group I) was less as compared to other group.
Twelve clinically healthy, adult male dogs randomly equally divided into two groups (I and II). Animals of both the groups received xylazine-ketamine anesthesia. Laparoscopic bilateral vasectomy was performed in group I, whereas in animals of group II in additition to vasectomy, spermatic artery-vein plexus were clipped with titanium clips at a distance of 1 to 2 cm. Insufflation of abdominal cavity was achieved by CO 2 (2 liter/minute) at 10 mm Hg pressure gradient. Clinical observations revealed no significant changes. Differential leukocyte count (DLC) revealed significant neutrophilia and comparative lymphopenia on 3rd postoperative in both groups. Significant increase (p < 0.05) in plasma alkaline and acid phosphatase level was observed on day 3 postoperatively. Indices of oxidative stress viz lipid peroxidation (LPO), catalase (CAT), superoxide dismutase (SOD), reduced glutathione activity and acute phase protein, ceruloplasmin level in plasma did not revealed any major significant changes but indicated that oxidative stress was more in group II animals. Plasma cortisol level increased significantly (p < 0.01) after operation and testosterone level showed gradual decrease (p > 0.05) up to 7th postoperative day in animals of group II. On the basis of the parameters studied, it can be concluded that capnoperitoneum at 10 mm Hg pressure gradient and CO 2 at the flow rate of 2 liter/minute provides optimum visualization of intra-abdominal organs and found suitable for laparoscopic sterilization in male dogs. The laparoscopic vasectomy alone in male dogs was found comparatively quick, less time consuming and can be successfully applied for mass sterilization program. Oxidative stress in laparoscopic vasectomy (group I) was less as compared to other group.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.