Objectives:To investigate the antiplatelet activity of alpha-lipoic acid (α-LA) and dihydroquercetin (DHQ).Materials andMethods: Antiplatelet activity of the α-LA and DHQ was evaluated in rich platelet plasma of rat. The platelet aggregation was induced by adenosine diphosphate (ADP) in concentration of 4 × 10-5 M.Results:α-LA and DHQ inhibited platelet aggregation in concentration-dependent manner. The antiplatelet activity of α-LA was more pronounced than DHQ. DHQ also increased the antiplatelet activity of α-LA by 1.4 times.Conclusion:Combined simultaneous use of α-LA and DHQ possessed the high antiplatelet activity, and DHQ potentiated the activity of α-LA.

Objectives:In this study, we have evaluated the influence of antioxidant complex (AOC) (dihydroquercetin and lipoic acid) on pool of nonadhesive leukocytes and leukocytes with low and high adhesion in chronic venous insufficiency (CVI) of lower limbs in rats.Materials and Methods:Model of CVI of lower limbs was created by blood flow restriction in caudal vena cava. AOC was administered at 100 mg/kg b.w./day for 14 days p.o. The venous pressure in vena cava and adhesion of leukocytes were estimated on the 14th day of the experiment.Results:On the 14th day, the venous pressure in groups of animals (experimental and control) was in 2.7-2.9-fold higher. In the control group, the significant increase of leukocytes pool with high adhesion was detected. Intragastric administration of AOC reduced the number of leukocytes with high adhesion and increased the number of nonadhesive leukocytes and leukocytes with a low adhesion.Conclusions:Model of CVI of lower limb is accompanied by increased venous pressure and raised adhesion activity of leukocytes. Administration of AOC for 14 days reduces the adhesive activity of leukocytes.

OBJECTIVES:The effect of course administration of amlodipine on whole blood viscosity and on macro- and microrheological parameters was evaluated.MATERIALS AND METHODS:SHRs were treated intragastrically with amlodipine at a dose of 10 mg/kg for 6 weeks. After finishing the course, hemodynamic and hemorheological parameters were measured.RESULTS:The antihypertensive treatment with amlodipine resulted in a significant decrease in mean blood pressure by 29% and left ventricular to body weight mass index by 7%. Nevertheless, BV tended to increase. The administration of amlodipine had no effect on PV, plasma fibrinogen concentration, RBC aggregation, and RBC deformability, but hematocrit was higher (by 6%) than it was in control group.CONCLUSIONS:These results demonstrate that amlodipine has no positive hemorheological improvements when administered to SHRs.