BackgroundAntioxidant molecules, which scavenge free radical species to prevent or delay oxidative damage of important macromolecules, membrane lipids and lipoproteins, are prevalent in plasma and other biological fluids. Among them, bilirubin, uric acid and protein thiols are the major endogenous antioxidants, while vitamins C and E, as well as a number of food-derived (poly)aromatic substances, belonging to stilbens, flavonoids and phenolic acids, are the main classes of nutritional antioxidants. Assays for total antioxidant capacity in plasma differ in their type of oxidation source, target and measurement used to detect the oxidized product.MethodsIn the present work we present an automated assay for the estimation of blood total antioxidant capacity (TAC assay), based on the crocin bleaching (oxidation) method. This method was adapted on a modern autoanalyzer, was linear over a wide range of values (0–3 mmol/L), and performed using an end point measurement.ResultsThe TAC method presented a linear correlation with another automated commercial Total Antioxidant Status (TAS) test. Detection of the interference of different metabolites revealed a significant participation of TAC from uric acid, bilirubin, albumin, a minor interference from ascorbic acid, and no interference from hemoglobin. TAC was not modified by two freeze/thawing cycles, and was stable in samples stored at room temperature for 4 hours. K-EDTA and heparin were the best anticoagulants, while citrate decreased TAC by 20%. Reference values derived from samples of normal blood donors was 1.175 ± 0.007 mmol/L (mean ± SEM), while a diet rich in antioxidants more than doubled this value.ConclusionsThe proposed TAC assay, is fully automated, stable and reliable, and could be of value in the estimation of the AC of plasma. It is further proposed to calculate the antioxidant capacity of plasma after a subtraction of all interference deriving from endogenous and/or exogenous metabolites. The antioxidant capacity of plasma thus calculated can be used as a useful indicator of the antioxidant value of foods and beverages in the daily diet.

Abstract:In an era governed by economic crisis, deep recession and lack of funds, the perspective of sustainable development drives local government to a more focused and constant effort to adapt to their daily requirements not only in respect to the central state but also towards the growing needs of the local society. As the need for planning actions in accordance with the principles of sustainable energy remains urgent, municipalities continue to envision and to respond to their role in helping to create a better future for generations to come. Thereupon the vision of the Municipality of Hersonissos, as firstly recorded in its Business Plan for 2011-2014, was, and still is, its' development in an Economic, Tourist and Cultural pole by adopting and applying the basic principles of sustainable development. At the same time the Municipality's rigid vision, planning, and evaluation, oriented by environmental protection, highlighted the need for networking on European level as although cities are different, their problems are often common. Thus on April 18, 2011, the Municipality of Hersonissos, joined the European Union initiative "Covenant of Mayors" [1] with the common aim of the fight against climate change. This article presents the significant initiatives taken in this direction in the last decade and their results not only on economical level but also along their social impact. It also aims to point out a municipality's vital role in knowledge and technology diffusion within the local society as sustainable energy development can be deployed not only by the private sector but also by the public.

Public local authorities are among the key players not only for assisting a nation to achieve its strategic energy goals but also for raising awareness towards relevant topics among its citizens. Yet, there has been little research on their role in this policy agenda. This paper aims to fill this gap by highlighting the significance of a local authority's outcomes, in the environmental sustainability sphere, by its participation in various projects and EU programs. The reference years are 2014 to 2019 and the focus area is energy savings in public buildings and raising awareness on energy issues and sustainable development. This article's aim is to outline the derived add value towards regional competitiveness and environmental sustainability from each action. Hersonisos Municipality's holistic approach aims to reduce its overall energy balance and to point out that local authorities can become both a beckon of sustainability and a regional development pillar.

In an era governed by economic