Modelo de indução de lesão no ligamento suspensório equino com utilização depunch para biópsia cutânea Suspensory ligament lesion model in horses using a skin biopsy punch RESUMO O objetivo deste estudo foi avaliar um novo modelo de lesão no ligamento suspensório (LS). INTRODUÇÃOO estudo das claudicações em equinos é uma das áreas de maior importância para a espécie, pois os problemas do sistema locomotor estão entre os mais comuns nos equinos. Entre as afecções mais frequentes, destaca-se a desmite do suspensório, uma enfermidade que acomete os membros torácicos e/ou pélvicos dos cavalos atletas. Grandes perdas econômicas ocorrem, pois o animal pode diminuir seu desempenho mesmo antes de os sinais clínicos se tornarem evidentes. Durante o longo período de tratamento, o animal necessita de repouso, ficando afastado de competições e treinamento. Alguns autores consideram que essa seja a maior causa de claudicação e perda da função nos cavalos atletas (HERTHEL, 2001
This study aimed to histologically evaluate the quality of tissue repair in equine suspensory ligament treated with two cell therapy protocols. All four limbs of six animals were operated simultaneously to remove a fragment in each ligament using a skin biopsy punch. Two days later, intralesional injections were performed using bone marrow mononuclear fraction (BM group), cultivated cells derived from adipose tissue (AT group), saline (positive control group), or no treatment (negative control group), in such way that each horse received all treatments. After sixty days biopsies were performed for histological analysis (H & E, Masson’s trichrome and picrosirius red) and immunohistochemistry analysis (collagen type III). Histological findings (H & E and Masson’s trichrome), birefringence intensity (through picrosirius) and collagen type III expression (through immunohistochemistry) were analyzed. Samples from treated groups had better birefringence intensity (P=0.007) and fiber alignment scores were superior compared to controls, though not statistically significant (P=0.08). Presence of inflammatory cells and intense staining for collagen type III occurred in all groups demonstrating an active healing process. In conclusion, both protocols resulted in improvement of tissue repair indicating their potential to be used as an adjuvant treatment of equine suspensory ligament disorders.
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