Um método estável, confiável, eficiente com relação a tempo e custo para detecção de açúcares redutores, baseado no método colorimétrico do ácido dinitrossalicílico (DNS), adaptado para placas de microtitulação em um banho de água modificado com tratamento de microondas é proposto. Além da influência benéfica sobre o meio ambiente devido à redução do volume dos reagentes utilizados, o método modificado é rápido e permite a análise de um número relativamente alto de amostras. Pode ser aplicado em diversos tipos de laboratórios, de processamento de alimentos à indústrias farmacêuticas. O método adaptado foi parcialmente validado (intervalo de linearidade entre 7 µg e 5 mg per poço; limite de detecção 3 µg per poço, desvio padrão em torno de 5% para curvas de calibração com glucose, e recuperação superior a 90%) e aplicado à séries de produtos contendo açúcares redutores, como amostras de mel e vinho e medicamentos que contêm lactose como excipiente. O método proposto é bem adaptado como uma técnica de alto rendimento para determinação de açúcares redutores, pois todas amostras analisadas deram resultados esperados de acordo com as suas notas técnicas.A sound, reliable, time-and cost-efficient method for detection of reducing sugars, based on the well known dinitrosalicylic acid (DNS) colorimetric method, adapted for microtiter plates, in a modified water bath with microwave treatment is proposed. Besides the benefic influence on the environment due to the reduction of the volume of the reagents, the modified method is rapid and allows the analysis of a relatively high number of samples. It can be applied in many types of laboratories from food processing to pharmaceutical industries. The adapted method was partially validated (linearity range between 7 µg and 5 mg per well; limit of detection 3 µg per well, standard deviation around 5% for calibration curves with glucose, and a recovery higher than 90%) and applied to a series of products containing reducing sugars, such as honey, wine samples and drugs containing lactose as an excipient. The proposed method is well-suited as a high-throughput technique for determination of reducing sugars as all the analyzed samples gave the expected results according with their technical notes.Keywords: dinitrosalicylic acid (DNS), microtiter plate assay, microwave supported reaction, reducing sugars IntroductionMonosaccharides, most of oligosaccharides, glucose polymers, such as starch and cellulose, and copolymers, like chitin, contain reducing ends. The measurements of the concentration of reducing ends can give valuable information about the analyzed sample. 1 Especially due to their presence in many natural products, reducing sugars (RS) have received considerable attention, which lead to a growing number of methods for their assay. The ability to measure the concentration of RS bears much significance for understanding the amount of sugar in foodstuffs, in beverages and even, although indirectly, the activities of some enzymes which are responsible for the hydrolysis of po...
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.