Considering the lack of research studies about nutrients' bioaccessibility in cashew apple, in this study the whole cashew apple juice and the cashew apple fibre were submitted to simulated in vitro gastrointestinal digestion. The samples were analysed before and after digestion and had their copper, iron, zinc, ascorbic acid, total extractable phenols and total antioxidant activity assessed. As a result, for the whole cashew apple juice, the content of copper and iron minerals bioaccessible fraction were 15% and 11.5% and for zinc this level was 3.7%. Regarding the cashew apple fibre, the bioaccessible fraction for these minerals was lower than 5%. The ascorbic acid, total extractable polyphenols and total antioxidant activity bioaccessible fraction for whole cashew apple juice showed bioaccessibility percentages of 26.2%, 39% and 27%, respectively, while for the cashew apple fibre, low bioaccessibles levels were found. The bioacessible percentage of zinc, ascorbic acid and total extractable polyphenols were higher in cashew apple juice than cashew apple fibre.
In Brazil, consumer preference of tropical native fruits has increased; however, many fruits are not commercially exploited, such as gabiroba (Campomanesia lineatifolia) and myrtle (Blepharocalyx salicifolius), from the Myrtaceae family. The present study aimed to evaluate the physical, physico-chemical, and functional characteristics of gabiroba and myrtle, harvested from native plants in the mountainous region of Ibiapaba-CE. The following characters were analyzed: pH, soluble solids, total acidity, total extractable polyphenols, ascorbic acid, and antioxidant activity by using the ABTS method. After pulping, the samples were divided into two groups. From the results, it was found that gabiroba had a low total acidity value (0.12 ± 0.01%) indicating a higher level of sweetness relative to myrtle. Myrtle presented values of longitudinal (0.94 cm) and transverse(1.35 cm) diameters, fresh weight (0.85g), pulp yield (68.0%), pH (4.5 ± 0.0), and total soluble solids (23.6 ± 1.7 °Bx) similar to those obtained in other studies. Bioactive compounds in gabiroba and myrtle included phenolics (GAE 229.37 ± 1.04and 511.65 ± 1.34mg/100g), ascorbic acid (74.44 ± 0.03 369.1 ± 0.28 mg/100g), and antioxidants (14.54 ± 1.0 and 16.05 ± 0.63 μ Mtrolox/g). Therefore, these fruits have the potential to be used as functional foods characterized by various health benefits. Keywords: Bioactive compounds. Functional foods. Native fruit. CARACTERIZAÇÃO FÍSICO-QUÍMICA DE GABIROBA (Campomanesia lineatifolia) E MURTA (Blepharocalyx salicifolius) NATIVAS DA REGIÃO SERRANA DE IBIAPABA-CE RESUMO -No Brasil os frutos nativos tropicais vêm conquistando a preferência dos consumidores, entretanto, muitos deles não é aproveitada comercialmente, a exemplo da gabiroba (Campomanesia lineatifolia) e Murta (Blepharocalyx salicifolius), pertencentes á família das Myrtaceae.Este trabalhou teve como objetivo avaliar as características físicas, físico-químicas e funcionais da gabiroba e murta, colhidas de plantas nativas na região serrana da Ibiapaba -CE, por meio das seguintes análises: pH, sólidos solúveis,acidez total, polifenóis extraíveis totais, ácido ascórbico e atividade antioxidante pelo método ABTS. Depois de despolpadas as amostras foram divididas em dois lotes. A partir dos resultados, verificou-se que entre os frutos analisados destacou-se por apresentar menor valor de acidez titulável (0,12 ± 0,01%) indicando um maior teor de doçura com relação às outras espécies. A Murta apresentou valores de diâmetro longitudinal (0,94 cm) e transversal (1,35 cm), massa fresca (0,85g), rendimento de polpa (68,0%), pH (4,5 ± 0,0) e sólidos solúveis totais (23,6 ± 1,7°Brix), próximos aos valores obtidos por outros autores referente a mesma espécie. Para os compostos bioativos foram obtidos valores de compostos fenólicos (229,37 ± 1,04 mg GAE/100 g ) ,(511,65 ± 1,34 mg GAE/100g), Ácido Ascórbico ( 74,44 ± 0,03 mg/100g) , (369,1 ± 0,28 mg/100g) e Capacidade Antioxidante (14,54 ± 1,0 µMol Trolox/g),(16,05±0,63 µMol Trolox/g), para gabiroba e murta , respectivamen...
Aspirin can reverse the Wnt-driven cystic phenotype in human organoids while reducing stem cell marker expression and increasing expression of Dickkopf-1, a Wnt antagonist often lost during CRC progression.BACKGROUND & AIMS: Aspirin reduces colorectal cancer (CRC) incidence and mortality. Understanding the biology responsible for this protective effect is key to developing biomarker-led approaches for rational clinical use. Wnt signaling drives CRC development from initiation to progression through regulation of epithelial-mesenchymal transition (EMT) and cancer stem cell populations. Here, we investigated whether aspirin can rescue these proinvasive phenotypes associated with CRC progression in Wnt-driven human and mouse intestinal organoids. METHODS:We evaluated aspirin-mediated effects on phenotype and stem cell markers in intestinal organoids derived from mouse (Apc Min/þ and Apc flox/flox ) and human familial adenomatous polyposis patients. CRC cell lines (HCT116 and Colo205) were used to study effects on motility, invasion, Wnt signaling, and EMT. RESULTS:Aspirin rescues the Wnt-driven cystic organoid phenotype by promoting budding in mouse and human Apc deficient organoids, which is paralleled by decreased stem cell marker expression. Aspirin-mediated Wnt inhibition in Apc-Min/þ mice is associated with EMT inhibition and decreased cell migration, invasion, and motility in CRC cell lines. Chemical Wnt activation induces EMT and stem-like alterations in CRC cells, which are rescued by aspirin. Aspirin increases expression of the Wnt antagonist Dickkopf-1 in CRC cells and organoids derived from familial adenomatous polyposis patients, which contributes to EMT and cancer stem cell inhibition. CONCLUSIONS:We provide evidence of phenotypic biomarkers of response to aspirin with an increased epithelial and reduced stem-like state mediated by an increase in Dickkopf-1. This highlights a novel mechanism of aspirin-mediated Wnt inhibition and potential phenotypic and molecular biomarkers for trials.
Mauritia flexuosa (Arecaceae), known as “Buriti,” is a Brazilian palm tree with high economic potential for local communities. Herein, we investigated the phytochemistry profile and antioxidant potential of M. flexuosa fruits and determined the bioaccessibility of phenolic compounds. Peels revealed upper values for phenols, flavonoids, carotenoids, tannins, and ascorbic acid when compared to the pulps and endocarps. All samples showed capacity to scavenger free radicals (0.5, 1.0, 2.0, 4.0, and 8.0 mg/mL) but peels presented higher scavenger action in all methods explored. Phenolic compounds identified by HPLC displayed reduced bioaccessibility after in vitro simulated gastrointestinal digestion for pulp (38.7%), peel (18.7%), and endocarp (22.3%) extracts (P < 0.05). Buriti fruits also protected rat blood cells against lysis induced by peroxyl radicals. We demonstrated the promising chemopreventive potentialities of M. flexuosa fruits and their by-products and peels with higher quantities of bioactive compounds and phenolic substances before and after in vitro bioaccessibility investigation. In Brazil, these parts are discarded or underused, mainly as feed for ruminant animals. Consequently, it is extremely important to explore nutritional characteristics of these by-products for human/livestock foods and to install biofriendly techniques and sustainable biotechnology handling of natural resources.
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