Background :Caffeine is one of the most widely consumed substances in the world. The main sources of caffeine in society are coffee or tea The cytotoxicity of caffeine may be due to its ability to trigger apoptosis Material and methods :A total number of 30 adult male albino rats (150-200 g) were used and were divided randomly into five equal groups (n=6 ) control group: 6 rats were I.P injected with sterile: Group II & IIa :12 rats were I. P. injected with single daily dose (2.5 mg/100g body weight/day) for three months this dose is equivalent to minimum human dose. Group IIa: 6 rats stopped treatment and left for one month after the period of treatment.Group III & IIIa: 12 rats were I.P. Injected with single daily dose (10 mg/100g body weight/day) for three months this dose is equivalent to maximum human dose. Group IIIa: 6 rats stopped treatment and left for one month after the period of treatment Samples from the submandibular salivary glands were fixed in 10% buffered neutral formalin and prepared routinely for paraffin sectioning and staining for histopathological changes and immuno-histochemical investigations of apoptotic cell antigen (Annexin A5).Results : Histopathological examination of caffeine treated rats revealed marked changes in glandular architecture, the acini became shrunkn with loss of their rnormal circular arrangement and their cytoplasm was vacuolated. Some serous cells degenerated and replaced by eosinophlic material. The nuclear changes appeared in form of (pleomorphism, hypertrophy, hyperchromatism and pyknosis), numerous normal and abnormal mitotic Figures were detected. Intercalated and excretory ducts were dilated. , blood vessels were dilated and congested. The immuno expression of Annexin A5 in the cytoplasmic basement membrane of the ductal cells was intense and highly significant. Changes were more aggressive in (group III). Recovery of some histological changes were observed especially in group received lower dose of caffeine . Conclusion:Excessive use of Caffeine markedly affect the histological structure of submandibular salivary gland. These changes could be reversible after caffeine stoppage if the doses is small while the histopathological changes of the gland is irreversible if the doses is large .
Odontogenic keratocyst (OKC), and ameloblastoma (AB) aggressive epithelial odontogenic tumors with a high recurrence rate though, their aggressive nature are not totally understood, Many epithelial tumors are characterized by stromal reaction, Myofibroblast is one of stromal component that could contribute to the biologic behavior of these lesions, Fifteen cases of AB and OKC were operated on under general anesthesia, all cases were subjected to immunohistochemical staining with alpha-smooth muscle actin and flow cytometry analysis, ameloblastoma showed expression of α muscle actin between the follicles as well as around the blood vessels, while in OKC the expression was mostly in connective tissue wall of cyst lining, as well as flow cytometry analysis showed tumors were diploid and showed a percentage of cells in S-phase higher than 26%. OKC had a higher value than AB but the values were close and statistically insignificant, we concluded that the immunohistochemical expression of MF in AB & OKC has insignificant difference may contribute to the similar proliferative potential of both AB& OKC, as well The high proliferation rate of OKC reinforces its classification as a benign odontogenic neoplasm rather than a cyst Objective To detect the proliferative potential of odontogenic keratocyst, versus ameloblastoma and their correlation to presence of stromal myofibroblasts (258)
BACKGROUND: Tongue cancer is one of the most common head and neck cancers in the world. Nowadays, natural compounds are important resources of many anti-cancer drugs. Venom from honey bees possesses potent anti-cancer activities. Cisplatin is a chemotherapeutic drug that has been used for decades to treat cancer cells. Recently, combination therapy has been a popular treatment choice for cancer patients. AIM: This study was conducted to evaluate the synergistic cytotoxic effect of honey bee venom (BV) and cisplatin on tongue squamous cell carcinoma 25 (SCC-25) cell lines. METHODS: The cytotoxic effect was determined using methyl thiazol tetrazolium assay, microscopic examination, real-time polymerase chain reaction (RT-PCR), and statistical analysis. RESULTS: The findings revealed that the cytotoxic potential of the tested drugs on SCC-25 cells was dose-dependent. Microscopic examination showed that BV and cisplatin alone and in combination mainly produced apoptotic cell death. Regarding RT-PCR results, P53 and caspase-3 expression levels were significantly increased in SCC-25-treated cells (p = 0.0001). CONCLUSION: The combined use of BV and cisplatin induced a marked synergistic cytotoxic effect on SCC-25 cell line.
Aim: This study examined the effect of cervical finish line preparation on the internal adaptation of all-ceramic crowns produced, using CAD CAM technology with two fabricated materials: IPs Emax and Zirconia-reinforced lithium silicate (Celtra Duo).Methods: Two intact human premolars were collected with similar preparation dimensions, except for a (1.00 mm) on the margin. For sample standardization, 40 epoxy replicas of prepared premolar teeth were produced. Deep chamfer (DC group, n = 20) and knife-edge (KE group, n = 20) finish lines were used to split samples. Each group was divided into two subgroups according to fabricated material. Subgroup 1: lithium di silicate ceramic (n=10) and Subgroup 2: lithium silicate reinforced with zirconia (n=10). All crowns were CAD/CAM-made. All samples were aged by thermo-cycling after being bonded to a tooth, and the internal adaptation was measured using a stereomicroscope. Results:The findings demonstrated an increase in the internal adaptation between vertical preparation (127.7±8.6) and deep chamfer (109.9±6.4), regardless of the material. In addition, regardless of methodology, the difference between E-max (119.6±16) and Celtra (118±5.7).Conclusions: Vertical preparation may be an alternative to horizontal preparation and more conservative to sound tooth structure throughout the fabrication of fixed abutments to give a less intrusive option.
Background: Osteosarcoma (OS) is the most common primary bone malignancy that affects children and adolescents. It is considered as a serious threat to the human health globally. For many years plants and natural constitutes components such as vitamin C (ascorbic acid), green tea have been used as anti-cancer drugs. Aim: The objective of this in vitro study was to investigate the effect of vitamin C, green tea and their combination on Mg-63 cell line. Material Methods: Treatment of cell line (Mg-63) by different concentrations of vitamin, green tea and their combination was done to evaluate the viability of the treated cells by SRB assay. Microscopic examination and were applied. Results: Regarding cytotoxicity effect of vitamin C, green tea and their combination on Mg-63cell line, it was noticed that cell distribution showed a variable arrest at different phase of cell division. Where there was nonsignificant difference of arrested cells of these drugs compared with its value in non-treated G0-G1 phase control cells while there was a significant elevated arrest of treated cells during the G2-M phase and the significant difference of cell arrest at G2-M phase was type of treatment related. Conclusion:From the results of the present study, we noticed that vitamin green tea and their combination have cytotoxic effect on Mg-63 cell line, it also induced an effect on the cell cycle distribution, resulting in apoptosis, necrosis and autophagy.
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