PCD is more effective than percutaneous needle aspiration in the management of liver abscess. Percutaneous needle aspiration can be used as a valid alternative for simple abscesses 50 mm in diameter or smaller.

Background High-throughput assays for the detection of SARS-CoV-2 variants of concern (VOC) and interest (VOI) are a diagnostic alternative when whole genome sequencing (WGS) is unavailable or limited. Objective This study evaluated the clinical and analytical performance of the Seegene Allplex TM SARS-CoV-2 Variants I assay, which detects the HV69/70 deletion, N501Y and E484K mutations of the S gene. Methods Genotyping was evaluated on 873 SARS-CoV-2 RNA positive specimens, 408 nasopharyngeal (NP) swabs and 463 saline gargle (SG) specimens, with WGS used as the reference standard. Analytical performance was assessed including stability, reproducibility, limit of detection (LOD), cross-reactivity and interference with various respiratory microorganisms. Results The clinical study revealed sensitivity of 100% (95% CI 99.27%-100%) and specificity of 100% (95% CI 98.99%-100%) for HV69/70 deletion, sensitivity of 100% (95% CI 99.55%-100%) and specificity of 100% (95% CI 93.73% - 100%) for N501Y, and sensitivity of 100% (95% CI 98.94% - 100%) and specificity of 98.10% (95% CI 96.53% - 99.08%) for E484K mutation. The E484Q mutation was detected in 10 specimens of the Kappa variant (B.1.627.1). Analytical performance demonstrated stability and reproducibility over 7 days, and LOD was calculated at 698 cp/mL for NP swab specimens, and 968 cp/mL for SG specimens. No interference or cross-reactivity with other microorganisms was noted. Conclusion The Allplex TM SARS-CoV-2 Variants I assay is acceptable for clinical use for the detection of variant of concern and variant of interest.

ntroduction: Cellulitis is acute skin infection and/or infection of subcutaneous tissue, mostly caused by Streptococcus pyogenes and Staphylococcus aureus. Clinical preview is usually obvious and enough for diagnosis. Tretment is antimicrobial therapy. In recurrent cases a prophylaxis is very often needed. Objectives: Analysis some of the epidemiological and clinical characteristics of cellulitis. Patients and methods: Retrospective analysis of medical documentation of patients with clinical preview of cellulitis who were hospitalized in Clinic for infective diseases of Clinical Center of University of Sarajevo in last three years. Results: In period of three years 123 patients were hospitalized with clinical preview of cellulitis in the broadest sense of the word. In 123 of cellulitises, 35/123 (28.45 %) were erisipelases-superficial type and 88/123 (71,55 %) were deep cellulitises. Men were more affected 56,09 %, average of age was 50.22 years. Before hospitalization patients had ambulance treatment in average of 5.12 days, and hospitalization was long in average of 13.33 days. Risk factors wich contributes to the disease were found in 71.54 % of cases. Due to localisation, skin disorders on lower limb were the most frequent 71.56 %, cellulitis of upper limb were found in 12.19 % , head and/or neck in 13.08 %, trunk in 3.25 %. Repetition of disease were found in 4.8 % in patients wtih risk factors. Bacteremic isolats were confirmed in 27.64 % of cases. In all patients empirical antibiotic treatment were started, in the 62.60 % the first choice of medicine was antibiotic from the group of lincosamides. Conclusion: Cellulitis is very serious disease that can be prevented.

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