Heptaminol Interferes in the AxSYM FPIA Amphetamine/Methamphetamine II Assay

Edno-Mcheik, Leila; Gaulier, Jean-Michel; Combourieu, I.; Lacassie, Éric; Hadzic, Amir

doi:10.1093/clinchem/47.8.1499

Cited by 3 publications

(2 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The chemical structures of these compounds were also downloaded (Figure 4A) from the ZINC database (http://zinc15.docking.org). Among these small compounds, previous studies have proven that heptaminol [44,45] and tranexamic acid [46] can be used in cardiovascular diseases, while the others have the effects of anti-tumor or antiinflammatory, etc.…”

Section: Candidate Anti-cardiotoxicity Compounds Via Cmap Analysismentioning

confidence: 99%

“…Meanwhile, our study also found that cardiac injury induced by AC exposure in zebrafish mainly included pericardial edema and atrial enlargement. Heptaminol was widely recognized for the treatment for right heart failure, coronary heart disease, and cardiogenic edema, which can improve atrioventricular node excitability, excite the conduction system, and restore its normal function [44,45,64].…”

Section: E924092-10mentioning

confidence: 99%

See 1 more Smart Citation

Potential Molecular Mechanisms and Drugs for Aconitine-Induced Cardiotoxicity in Zebrafish through RNA Sequencing and Bioinformatics Analysis

et al. 2020

View full text Add to dashboard Cite

Background:Accumulating evidence suggests that cardiotoxicity is one of the main manifestations of aconitine (AC) poisoning. However, the molecular mechanism of AC-induced cardiotoxicity remains unclear, there is little direct evidence for therapeutic targets and drugs of AC-induced cardiotoxicity. Material/Methods:Zebrafish were exposed to AC to evaluate cardiotoxicity by calculating the heart rates and observing the changes of cardiac and vascular structure. RNA-seq (RNA sequencing) and bioinformatics analysis were used to obtain differentially expressed genes (DEGs). The anti-AC cardiotoxicity compound was identified via connectivity map (CMAP) analysis and molecular docking. Results:AC-induced cardiotoxicity in zebrafish predominantly included arrhythmias, extended sinus venous and bulbus arteriosus (SV-BA) distance, and larger pericardial edema aera. A total of 1380 DEGs were identified by RNA-seq and bioinformatics analysis. cyclin-dependent kinase-1 (CDK1) was screened as the hub gene and the most potential therapeutic target due to its significant downregulation in cardiotoxicity based on protein-protein interaction (PPI) and drug-gene interaction (DGIdb) network analysis. Cell cycle signal pathway was the most significant pathways identified in the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. Furthermore, the expression of CDK1 was validated in the Gene Expression Omnibus (GEO) database GSE71906, GSE65705, and GSE95140. Finally, heptaminol was identified as a novel anti-AC cardiotoxicity compound via CMAP analysis and molecular docking. Conclusions:Totally, hub genes and key pathways identified in this study can aid in the understanding of the molecular changes in AC-induced cardiotoxicity. Meanwhile, we provide a systematic method to explore drug toxicity prevention and treatment.

show abstract

Section: Candidate Anti-cardiotoxicity Compounds Via Cmap Analysismentioning

confidence: 99%

Section: E924092-10mentioning

confidence: 99%

Potential Molecular Mechanisms and Drugs for Aconitine-Induced Cardiotoxicity in Zebrafish through RNA Sequencing and Bioinformatics Analysis

et al. 2020

View full text Add to dashboard Cite

show abstract

Screening for and validated quantification of amphetamines and of amphetamine‐ and piperazine‐derived designer drugs in human blood plasma by gas chromatography/mass spectrometry

et al. 2003

View full text Add to dashboard Cite

The classical stimulants amphetamine, methamphetamine, ethylamphetamine and the amphetamine-derived designer drugs MDA, MDMA ('ecstasy'), MDEA, BDB and MBDB have been widely abused for a relatively long time. In recent years, a number of newer designer drugs have entered the illicit drug market. 4-Methylthioamphetamine (MTA), p-methoxyamphetamine (PMA) and p-methoxymethamphetamine (PMMA) are also derived from amphetamine. Other designer drugs are derived from piperazine, such as benzylpiperazine (BZP), methylenedioxybenzylpiperazine (MDBP), trifluoromethylphenylpiperazine (TFMPP), m-chlorophenylpiperazine (mCPP) and p-methoxyphenylpiperazine (MeOPP). A number of severe or even fatal intoxications involving these newer substances, especially PMA, have been reported. This paper describes a method for screening for and simultaneous quantification of the above-mentioned compounds and the metabolites p-hydroxyamphetamine and p-hydroxymethamphetamine (pholedrine) in human blood plasma. The analytes were analyzed by gas chromatography/mass spectrometry in the selected-ion monitoring mode after mixed-mode solid-phase extraction (HCX) and derivatization with heptafluorobutyric anhydride. The method was fully validated according to international guidelines. It was linear from 5 to 1000 micro g l(-1) for all analytes. Data for accuracy and precision were within required limits with the exception of those for MDBP. The limit of quantification was 5 micro g l(-1) for all analytes. The applicability of the assay was proven by analysis of authentic plasma samples and of a certified reference sample. This procedure should also be suitable for confirmation of immunoassay results positive for amphetamines and/or designer drugs of the ecstasy type.

show abstract

Amphetamine-derived Designer Drugs: Metabolism and Screening Procedures/Amphetamin-verwandte Designer-Drogen: Metabolismus und Screening Verfahren

Maurer¹,

Kræmer²

2002

LaboratoriumsMedizin

View full text Add to dashboard Cite

Heptaminol Interferes in the AxSYM FPIA Amphetamine/Methamphetamine II Assay

Cited by 3 publications

References 3 publications

Potential Molecular Mechanisms and Drugs for Aconitine-Induced Cardiotoxicity in Zebrafish through RNA Sequencing and Bioinformatics Analysis

Potential Molecular Mechanisms and Drugs for Aconitine-Induced Cardiotoxicity in Zebrafish through RNA Sequencing and Bioinformatics Analysis

Screening for and validated quantification of amphetamines and of amphetamine‐ and piperazine‐derived designer drugs in human blood plasma by gas chromatography/mass spectrometry

Amphetamine-derived Designer Drugs: Metabolism and Screening Procedures/Amphetamin-verwandte Designer-Drogen: Metabolismus und Screening Verfahren

Contact Info

Product

Resources

About