Background: Endodontic treatment involves the removal of the vital and necrotic contents of the root canal through chemo-mechanical means followed by obturation of the prepared root canal to prevent the ingress of fluids and avoid bacterial infection or regrowth. Root canal sealers and core filling materials are used together to fill the irregularities in the root. Penetration into the dentinal tubules also results in the inhibition of bacterial regrowth and increases the success of root canal therapy. Aim: This study aimed to evaluate the penetration depth of various sealers into the dentinal tubules using a confocal microscope. Materials and methods: A total of 65 specimens were decoronated to standardize the root length of 13mm. Working length was determined, and Biomechanical preparation for all the samples was done with a rotary ProTaper file till F4. Samples were randomly divided into five groups containing 13 teeth in each group based on the sealer used, namely Group 1: Endomethasone (n=13), Group 2: AH-Plus (n=13), Group 3: Roekoseal (n=13), Group 4: MTA Fillapex (n=13), Group 5: Endosequence BC (n=13). All the sealers were labelled with Rhodamine-B dye, and samples were obturated using cold lateral compaction technique. The specimens were sectioned orthogonally at coronal, middle, and apical thirds. All the samples were examined with a Zeiss Pascal Laser Scanning Microscope to examine the sealer penetration depth into the dentinal tubules. The data were subjected to statistical analysis using one-way Analysis of Variance (ANOVA) and Tukey's Honest Significant Difference (HSD) tests. Results: Endosequence BC showed the highest penetration into dentinal tubules, followed by MTA Fillapex and Roekoseal, AH-Plus, and Endomethasone exhibited the least penetration. Conclusion: Endosequence BC sealer exhibited maximum penetration. All the groups showed maximum penetration at coronal third, followed by the middle and apical third.
Introduction: The mandibular first molar poses a series of anatomic challenges such as multiple canals, isthmuses, lateral canals, and apical ramifications. Additionally, the distal surface of mesial root presents a thin zone, which is 4-6 mm cervical to canal orifice with a thin area of dentin termed as “danger zone,” as it increases the risk of perforation of furcal dentin during mechanical instrumentation. Aim: To measure the amount of the dentin removed after biomechanical preparation with XP-Endoshaper and Neoendo flex files in the danger zone of the mandibular first molar using CBCT. Methodology: Twenty-four mandibular first molars, the mesial roots of which presented a curvature of 20-30 degrees, were selected using the Schneider method. Samples were instrumented with Neoendo rotary file system (n=12) and XP-Endoshaper rotary single file system (n=12). The samples were scanned using CBCT before and after instrumentation. The analyzed parameter includes the remaining dentin thickness at the danger zone of the mandibular 1st molar. The obtained data were subjected to statistical analysis. Results: There was no significant difference in remaining dentine thickness between the two rotary systems (p > 0.05). Conclusion: XP-Endoshaper revealed better results than Neoendo rotary files. However, both rotary file systems showed no significant difference in the remaining dentin thickness in the danger zone of the mandibular first molar. Keywords: Danger zone, Remaining dentine thickness, XP EndoShaper, NeoEndo, CBCT.
Background:The most desirable outcome of endodontic treatment is to achieve a fluid-tight seal of the root canal space. Root canal sealers are used in combination with core filling materials to fill the irregularities in the root canal, resulting in a three-dimensional seal that prevents bacterial regrowth. Aim: This study was aimed to compare the apical sealing ability of three root canal sealers AH Plus, Endosequence BC, and Endoseal MTA, using a single cone gutta-percha obturation technique. Materials and methods: Forty extracted human single-rooted mandibular premolar teeth were decoronated to a standardized length of 15 mm and instrumented using crown down technique with the ProTaper gold rotary file system to apical file size F3. The roots were randomly allocated into three experimental groups (n=10) and two control groups (n=5). All the samples in experimental groups were obturated with a matched taper single cone: Group 1, Group 2 and Group 3 were obturated using AH Plus, Endosequence BC and Endoseal MTA sealers, respectively. Samples were immersed in 1% Methylene blue dye solution for 72 hours, and then the roots were split longitudinally and observed under a stereomicroscope. Apical microleakage was measured from the apex to the most coronal level of dye penetration. The data obtained were subjected to statistical analysis. Results: Samples in all the groups showed evidence of leakage, except in the negative control group. One-way ANOVA showed significant differences between the groups (p=0.00132). Posthoc analysis exhibited a significant difference between group 2 and group 3 (p=0.0102). Conclusion: Endosequence BC showed a superior seal and less microleakage compared to the two other sealers used in this study using a single cone guttapercha obturation technique.
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