ABSTRAKAncaman global pada kasus Methicillin Resistant Staphylococcus aureus (MRSA) membutuhkan alternatif penanganan dengan tanaman obat tradisional. Bakteri endofit pada tanaman pegagan (Centella asiatica) memiliki kemampuan menghasilkan senyawa metabolit sekunder bersifat antibakteri yang serupa dengan tanaman inangnya. Tujuan penelitian ini untuk mengetahui aktivitas antibakteri isolat bakteri endofit daun pegagan (C. asiatica) terhadap S. aureus. Penelitian ini bersifat deskriptif yaitu isolat bakteri endofit daun pegagan (C. asiatica) diujikan dengan metode difusi cakram terhadap S. aureus. Isolat yang paling berpotensi memiliki aktivitas antibakteri dilakukan uji metabolit untuk mengetahui senyawa antibakteri yang dihasilkan. Identifikasi bakteri endofit berdasarkan morfologi koloni, morfologi sel, dan uji biokimia. Hasil penelitian menunjukkan 2 dari 37 isolat memiliki aktivitas terhadap S. aureus dengan zona hambat sebesar 9,02 mm dan 15,9 mm. Isolat yang paling berpotensi memiliki aktivitas tertinggi yaitu isolat I2 dengan zona hambat sebesar 15,9 mm. Isolat I2 memiliki kemiripan dengan genus Bacillus dan kemampuan mengasilkan senyawa antibakteri seperti alkaloid, saponin dan terpenoid. Kata kunci: antibakteri; bakteri endofit; Centella asiatica; Staphylococcus aureus ABSTRACTAntibacterial activity of endophytic bacteria isolate from pegagan leaves (Centella asiatica) against Staphylococcus aureusThe global threat in the case of Methicillin Resistant Staphylococcus aureus (MRSA) requires alternative treatment using traditional medicinal plants. Endophytic bacteria found in Pegagan plants (Centella asiatica) have ability to produce secondary metabolites with antibacterial capabilities similar to their host plants. The purpose of this study is to determine the antibacterial activity of endophytic bacterial isolates of Pegagan (C. asiatica) against S. aureus. This study is a descriptive research where endophytic bacterial isolates of Pegagan leaves (C. asiatica) were tested with disk diffusion method against S. aureus. The most potential isolates with antibacterial activity were performed metabolites test to determine the antibacterial compounds produced. Endophytic bacteria identification based on colony morphology, cell morphology and biochemical tests. The results showed that 2 out of 37 isolates had activity against S. aureus with inhibition zone of 9,02 mm and 15.9 mm. The most potential isolate that has highest activity was I2 isolate with inhibition zone of 15.9 mm. Isolate I2 has similarities with the genus Bacillus and the ability to produce antibacterial compounds such as alkaloids, saponins and terpenoids. Key words: antibacterial; Centella asiatica; endophytic bacteria; Staphylococcus aureus
Background: Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), is an emerging disease that has become pandemic. Healthcare workers are vulnerable persons due to SARS-CoV-2 infection. Healthcare workers are a government priority in the COVID-19 vaccine program. Antibody titer evaluation to healthcare workers as the first liner is essential due to the risk of the job. Purposes: The study aims to assess IgG antibody humoral response in healthcare workers in the emergency unit of Anton Soedjarwo Hospital, Pontianak, before and after the third dose of the COVID-19 vaccine. Methods: This study was a descriptive study with a cohort method. Twenty-two healthcare workers in the Emergency Unit in Anton Soedjarwo Hospital, Pontianak, participated in the study. Three ml venous blood samples were collected from the participants in two periods to quantify IgG antibody titer. The first period was before the third dose of the COVID-19 vaccine, and the second period was one month after the third dose of the COVID-19 vaccine. Chemiluminescent Microparticle Immunoassay (CMIA) methods were used to quantification IgG antibodies against spike-receptor binding domain (S-RBD) protein of SARS-CoV-2 Results: One month before the third dose of the COVID-19 vaccine, the majority of IgG antibodies show seropositivity (90.91%), with most on range 50-10,000 AU/ml (54.55%). Two participants (9.09%) show IgG antibodies less than 50 AU/ml (seronegative). One month after the third vaccination dose, all participants (100%) elicit IgG antibodies. Based on the comparison of IgG antibody titer before and after the third dose of the vaccine, the majority (68.18%) showed increasing the IgG antibody titer after the third vaccine. Conclusion: The third dose vaccine might effectively elevate immune response to SARS-CoV-2 infection.
ABSTRAKPropionibacterium acnes merupakan penyebab utama terjadinya acne vulgaris. Pengobatan tanda, gejala, dan kekambuhan acne vulgaris cukup sulit dikarenakan P. acnes yang resisten dengan antibakteri seperti golongan linkosamida. Kunyit (Curcuma longa L.) mempunyai senyawa aktif kurkumin yang merupakan polifenol alami dengan sifat antibakteri terhadap P. acnes. Bakteri endofit yang diisolasi dari suatu tanaman dapat menghasilkan metabolit sekunder yang sama dengan tanaman aslinya. Penelitian bertujuan untuk mengetahui aktivitas antibakteri metabolit sekunder endofit kunyit terhadap P. acnes. Penelitian bersifat deskriptif yaitu isolat bakteri endofit kunyit diujikan dengan metode difusi cakram terhadap P. acnes. Isolat yang paling berpotensi memiliki aktivitas antibakteri diidentifikasi berdasarkan karakterisasi morfologi koloni, morfologi sel, biokimia dan dilakukan uji senyawa metabolit sekunder dengan menggunakan metode Ciulei. Sebanyak 12 dari 17 isolat memiliki aktivitas terhadap P. acnes dengan diameter zona hambat terbedar yaitu 21,2 mm dengan kode isolat H5. Hasil identifikasi menunjukkan bahwa H5 memiliki kemiripan dengan genus Bacillus. Hasil uji metabolit menunjukkan bahwa senyawa antibakteri yang dihasilkan isolat H5 yaitu saponin, terpenoid dan flavanoid. Bakteri endofit tanaman kunyit memiliki potensi sebagai antibakteri terhadap bakteri P. acnes. Kata kunci: antibakteri; bakteri endofit; Curcuma longa L.; Propionibacterium acnes. ABSTRACTAntibacterial activity of secondary metabolites of endophytic bacterial isolated from tumeric (Curcuma longa L.) against Propionibacterium acnes. Propionibacterium acnes is the main cause of acne vulgaris. Treatment to control the signs, symptoms, and recurrence of acne vulgaris is not easy due to its resistance to antibacterials such as linkosamide group. Turmeric (C. longa L.) has an active compound, curcumin, the main natural polyphenol which has antibacterial properties against P. acnes. Endophytic bacteria isolated from plant can produce same secondary metabolites with the host plant. The study aimed to determine the antibacterial activity of turmeric bacterial endophytes secondary metabolites against P. acnes. The research was descriptive research where endophytic bacterial isolates of turmeric were tested by disk diffusion method against P. acnes. The most potential isolates with antibacterial activity was identified based on the characterization of colony morphology, cell morphology, biochemical tests and a secondary metabolic compound test used Ciulei method. A total of 12 from 17 isolates had activity against P. acnes with the biggest inhibition zone is 21.2 mm with code H5. The identification results showed that H5 had similarities with the genus Bacillus. Metabolite test showed that the antibacterial compounds produced by H5 were saponins, terpenoids and flavonoids. The endophytic bacteria of turmeric plant has potential as an antibacterial against P. acnes. Keywords: antibacterial; endophytic bacteria; Curcuma longa L.; P. acnes.
Tujuan: Penelitian ini bertujuan untuk mengetahui karakter bakteri Gram negatif endofit tanaman kunyit yang memiliki kemampuan Quorum Quenching (QQ). Metode: Bakteri Gram negatif endofit tanaman kunyit diisolasi, dimurnikan, dan disubkultur pada media NA dengan metode cawan gores. Uji QQ dilakukan dengan metode disc diffusion untuk mengukur pembentukan zona hambat pada bakteri uji Chromobacterium violaceum. Bakteri yang memiliki kemampuan QQ dikarakterisasi berdasarkan morfologi koloni, morfologi sel dan aktivitas biokimia. Hasil: Sebanyak 21 isolat bakteri endofit berhasil diisolasi dan dimurnikan dari tanaman kunyit dan 14 isolat diantaranya merupakan bakteri Gram negatif. Semua isolat bakteri Gram negatif endofit memiliki kemampuan QQ yang berkisar antara 6,5-13,5 mm. Satu isolat yang memiliki kemampuan QQ paling besar adalah isolat 6 dengan hasil identifikasi termasuk ke dalam genus Pseudomonas. Simpulan:Bakteri Gram negatif endofit tanaman kunyit yang memiliki kemampuan QQ paling potensial adalah genus Pseudomonas.
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