P rObIOtICS play an effective and significant role in human health. The aim of this study was to isolate potential probiotics from Egyptian sources. Among the 14 different bacterial isolates recovered from 8 different sources on Man-rogosa-Sharp (MrS) agar medium, 5 isolates exhibited tolerance to pH 3 and survived at bile concentration of 0.3% for 3h. the selected isolates were resistant to amikacin, vancomycin, ciprofloxacin and bacitracin. In addition, they showed high antibacterial activity against 7 pathogens. Hydrophobicity using xylene and toluene showed high hydrophobic property for M isolate with toluene and xylene (80.43±0.95% and 78.2±0.73%, respectively). The potent isolate was identified by 16S rRNA gene as Lactobacillus plantarum strain M (KY508300). this strain was able to remove 71.28 ± 0.4 % of lead (1mg/ml) after 5min. the probiotic strain M showed count stability in yoghurt up to 21 days. the characteristic features of L. plantarum strain M as potent probiotics entitled it to be used in industrial and environmental applications.
Exposure to ultraviolet (UV) radiation is one of the major factors that causes skin aging, erythema, sunburns, and skin cancer. This study aimed to select probiotic bacterial isolates able to produce high yield of hyaluronic acid (HA) to be employed for skin photoprotection and other possible biological applications. The selected isolates K11 and St3 were able to produce the highest yields of HA 4.8 and 4.4 mg/ml, respectively. Both isolates were identified as Enterococcus durans strain K11 and Lactiplantibacillus plantarum strain St3 using 16S rRNA gene sequencing. The antioxidant activity of HA produced by E. durans strain K11 and L. plantarum strain St3 was (65.4 0.2%) and (66.6 0.1%), respectively. The viability of UVB-irradiated keratinocytes pre-treated with HA produced by E. durans strain K11 and L. plantarum strain St3 was 91.3 and 91.4%, respectively, compared with the control. While the viability of UVB-irradiated keratinocytes post-treated with HA produced by E. durans strain K11 and L. plantarum strain St3 was 86 and 88.5%, respectively. To the best of our knowledge, this is the first recordation of HA production by Enterococcus durans and Lactiplantibacillus plantarum which revealed a significant radioprotection of the human keratinocytes against UVB radiation.
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