P rObIOtICS play an effective and significant role in human health. The aim of this study was to isolate potential probiotics from Egyptian sources. Among the 14 different bacterial isolates recovered from 8 different sources on Man-rogosa-Sharp (MrS) agar medium, 5 isolates exhibited tolerance to pH 3 and survived at bile concentration of 0.3% for 3h. the selected isolates were resistant to amikacin, vancomycin, ciprofloxacin and bacitracin. In addition, they showed high antibacterial activity against 7 pathogens. Hydrophobicity using xylene and toluene showed high hydrophobic property for M isolate with toluene and xylene (80.43±0.95% and 78.2±0.73%, respectively). The potent isolate was identified by 16S rRNA gene as Lactobacillus plantarum strain M (KY508300). this strain was able to remove 71.28 ± 0.4 % of lead (1mg/ml) after 5min. the probiotic strain M showed count stability in yoghurt up to 21 days. the characteristic features of L. plantarum strain M as potent probiotics entitled it to be used in industrial and environmental applications.
Exposure to ultraviolet (UV) radiation is one of the major factors that causes skin aging, erythema, sunburns, and skin cancer. This study aimed to select probiotic bacterial isolates able to produce high yield of hyaluronic acid (HA) to be employed for skin photoprotection and other possible biological applications. The selected isolates K11 and St3 were able to produce the highest yields of HA 4.8 and 4.4 mg/ml, respectively. Both isolates were identified as Enterococcus durans strain K11 and Lactiplantibacillus plantarum strain St3 using 16S rRNA gene sequencing. The antioxidant activity of HA produced by E. durans strain K11 and L. plantarum strain St3 was (65.4 0.2%) and (66.6 0.1%), respectively. The viability of UVB-irradiated keratinocytes pre-treated with HA produced by E. durans strain K11 and L. plantarum strain St3 was 91.3 and 91.4%, respectively, compared with the control. While the viability of UVB-irradiated keratinocytes post-treated with HA produced by E. durans strain K11 and L. plantarum strain St3 was 86 and 88.5%, respectively. To the best of our knowledge, this is the first recordation of HA production by Enterococcus durans and Lactiplantibacillus plantarum which revealed a significant radioprotection of the human keratinocytes against UVB radiation.
Plant probiotics are live microbial cells or cultures that support plant growth and control plant pathogens through different mechanisms. They have various effects on plants, including plant growth promotion effects through the production of indole acetic acid (IAA), biological control activity, and production of cellulase enzyme, thus induction of systemic resistance and increasing the availability of mineral elements. The present work aims at studying the potentials of Achromobacter xylosoxidans and Bacillus safensis as plant probiotics for the field cultivation of potatoes. In vitro studies of A. xylosoxidans and B. safensis have proved their abilities to produce IAA, and cellulase as well as exhibited antimicrobial activity against two plant pathogens that infect Solanum tuberosum as Fusarium oxysporum and Ralstonia solancerium under different conditions at a broad range of temperatures and pHs. Probiotic isolates A. xylosoxidans and B. safensis were able to tolerate (ultraviolet) Radiation exposure for up to two hours, the dose-response curve exhibited that the D10 values of A. xylosoxidans and B. safensis were 28 and 16 respectively. The in vivo study of the effects of probiotics A. xylosoxidans and B. safensis on S. tuberosum grown in sandy loamy soil was detected after cultivation for 60 days. The addition of both probiotic isolates either broth or wheat separately has enhanced all the growth parameters, however, the better results and increased production was in favor of adding probiotics with broth more than wheat. In the case of adding both probiotics with broth, the shoot dry weight was increased from 28 ± 1.0 in the control to 50 ± 1.2 gm, shoot length increased from 24 ± 1 to 45.7 ± 2 cm, branches numbers increased from 40 ± 4 to 70 ± 5, leaves number increased from 99 ± 6 to 130 ± 5, root dry weight increased from 9.3 ± 1.7 to 12.9 ± 2 gm, root length increased from 24 ± 3.8 to 35.7 ± 2 cm, tuber weight increased from 15 ± 2.3 to 37.0 ± 2 gm and tuber number increased from 9 ± 1 to 24.4, and the shoot and root rot percentage was reduced to 0%. On the other hand, both probiotics showed a remarkable protective effect against potato pathogens separately and reduced the negative impact of the infection using them together.
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