aulownia (Paulownia tomentosa L) is the one useful trees in China. All parts of paulownia tree (leaves, flowers, wood, bark, roots and seeds) have been used for many medicinal and industrial purposes. Buds were cultured on ¾ Murashige and Skoog (MS) solid medium supplemented with 0.2 mg/l benzyl adenine and 0.1 mg/l kinetin. The survival percentage and mean shoot length of irradiated plantlets with gamma radiation doses of 10, 20, 30, 40 and 50 Gy were calculated after eight weeks. The results showed a decrease in the survival percentage and mean shoot length by increasing gamma radiation doses. The lethal dose was 50 Gy and the optimal dose for explant survival was 10 Gy. Anatomical and ultrastructure of un-irradiated and irradiated paulownia were examined based on photonic and electron microscopy for plantlets leaves after 16 days from culturing. Anatomical studied of leaf surface showed variations in epidermal cells, trichomes, stomata and mesophyll cells. The ultrastructure sections showed formation of plastoglobule and starch granules which indicates a reduced carbon metabolism at sublethal dose of 40 Gy.
aulownia (Paulownia tomentosa) is an economically important tree, which is used in the manufacture of paper and furniture industry. Moreover, it considered a medicinal plant used for treating a diversity of diseases. Buds were cultured on Murashige and Skoog (MS) solid medium supplemented with benzyl amino purine (BAP) and kinetin (KIN). The resulting plantlets were cultured on MS medium containing different sodium chloride (NaCl) concentrations (50, 100 and 150 mM). The survival percentage and mean shoot length of plantlets were calculated after eight weeks. The results revealed that the survival percentage and mean shoot length were decreasing with increasing NaCl concentration. In order to provide a reference for investigating the mechanism of salinity resistance, variations of anatomy and chloroplast ultrastructure in the leaves of unstressed and stressed plantlets (50 and 150 mM NaCl) after 16 days from culture were examined under photonic and electron microscopy. The results showed that salinity treatments induced variations in leaf mesophyll, changes in vascular bundle, deformed trichomes, and appearance of starch granules in chloroplasts. The diversity in phenotypic and ultrastructure of leaves considered as genetic markers of paulownia plantlets against salinity.
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