Antibacterial and anti-inflammatory activities of methanolic extract of Juglans regia L. leaves (Walnut) grown in Libya were investiagted. Hot extraction method by methanol using Soxhlet apparatus was utilized.The antimicrobial effect was studied using agar diffusion method. Human red blood cell membrane stabilization (HRBC) method was used to estimate the anti-inflammatory properties of the leave extract. The extract of leaves exhibited antimicrobial activity against Staphylococcus aureus, and Candida albicans with a minimum inhibitory concentration (MIC) of 25 and 50 mg/ml, respectively. In addition, the extract concentrations of 20, 40, and 100 μg/mL inhibited lysis of HRBC with values of 25.8%, 35.5%, and 70%, respectively. The results showed that the methanolic extract of Juglans regia leaves exhibited antibacterial activity against S. aureus, and antifungal activity against C. albican, and produced anti-inflammatory activity.
This study aimed to evaluate the anti‐inflammatory activity of Dicliptera bupleuroides methanol extract (DBM) and its fractions. The samples showed significant inhibition of cyclooxygenase where DBM showed the highest inhibitory potential at 100 µg/mL estimated by 67.86%.. At a 400 mg/kg dose, all the samples showed pronounced results in carrageenan induced acute inflammation in rat model with DBM showed the highest efficiency displaying 65.32% inhibition compared to the untreated rats. Formalin model was also employed, DBM exhibited 65.33% and 69.39% inhibition at 200 and 400 mg/kg, respectively approaching the standard drug. High performance liquid chromatography revealed the presence of caffeic acid, gallic acid, quercetin and myricetin in DBM. Gas chromatography coupled with mass chromatography analysis of its n‐hexane fraction revealed the presence of 16 compounds belonging to fatty acids and sterols accounting for 85.26% of the total detected compounds. Molecular docking showed that hexadecanoic acid followed by decanedioic acid had the best fitting within cyclooxygenase‐II while nonacosane followed by hexatriacontane possessed pronounced fitting within the 5‐lipoxygenase active site. ADMET/ TOPKAT prediction concluded that most of the detected compounds showed reasonable pharmacokinetic, pharmacodynamic and toxicity properties that could be further modified to be more suitable for incorporation in pharmaceutical dosage forms.
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