RESUMO: Estudos prévios têm demonstrado alterações radiográficas em cascos de equinos obesos. Os objetivos foram estudar, com avaliação radiográfica, a relação espacial entre estojo córneo e falange distal de éguas jovens da raça Campolina com e sem obesidade. Foram utilizadas 22 éguas entre três e cinco anos de idade, sendo analisadas varáveis de adiposidade e medidas radiográficas dos cascos dos membros torácicos de éguas com escore corporal de 5 a 7/9 (Grupo Controle) e de 8 a 9 (Grupo Obeso). Foram feitas comparações entre os grupos e correlacionaram-se as variáveis de adiposidade entre si, variáveis adiposidade com variáveis casco e variáveis de casco entre si. Utilizou-se o teste t de Student para variáveis paramétricas e o teste Mann-Whitney para as não-paramétricas, para as medidas de correlação, utilizou o teste de Pearson para duas amostras paramétricas e o teste de Spearman para comparações que envolvam pelo menos uma variável não paramétrica (P<0,05). Os resultados demonstraram que éguas Campolinas obesas, ainda jovens, já apresentam indícios de alteração na relação espacial entre estojo córneo e falange distal.
IntroductionMesenchymal stem cells (MSCs) are defined as a population of multipotent cells able to differentiate and produce any cell type needed in a repair process, such as osteoblasts, chondroblasts, neurons, epithelial cells, and cardiac cells (1,2).This cell type has become the focus of numerous studies worldwide for providing clinically promising perspectives for cell therapy and also for its immunomodulatory potential (3,4), although the mechanisms of immunosuppression on inflammatory response and the mechanisms of transplant rejection are not fully elucidated (5).Recent studies have described the use of allogeneic and autologous MSCs for the repair of various tissues (4,6). However, there is little research involving xenotransplantation in animals and most of them only evaluated the cellular interaction in vitro between MSCs and T lymphocytes.Because of the great therapeutic potential of MSCs, in addition to the persistent doubts about their immunosuppressive capacity in vivo, further studies are needed to investigate the real potential of xenogenic transplantation using these cells for tissue repair in animals. Therefore, this study evaluated clinical and radiographic aspects of xenogenic transplantation of rat bone marrow-derived MSCs for the repair of radial bone defects created in rabbits.
Materials and methodsThis study was approved by the Institutional Animal Care Committee (protocol 97/2010).
Cellular cultureA total of five male, 4-week old Wistar rats were euthanized using anesthetic overdose. The animals were immersed in alcohol 70° to ensure antisepsis for cell collection and were taken to the laminar flow cabinet. The femurs were disarticulated and removed aseptically. The distal epiphyses were cut and the medullary canal was flushed with Dulbecco's modified Eagle's medium (DMEM, Gibco, Grand Island, NY, USA) with low glucose, containing 10% fetal bovine serum (FBS) (Gibco), 50.0 mg L -1 gentamicin,
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